Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1364478..1365042 Replicon chromosome
Accession NZ_CP094946
Organism Lacticaseibacillus paracasei strain VHProbi F11

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag MUB19_RS07015 Protein ID WP_016363448.1
Coordinates 1364740..1365042 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag MUB19_RS07010 Protein ID WP_003570051.1
Coordinates 1364478..1364747 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB19_RS06980 (MUB19_06955) 1359483..1360370 - 888 WP_003570041.1 ABC transporter ATP-binding protein -
MUB19_RS06985 (MUB19_06960) 1360567..1361793 - 1227 WP_003570043.1 hypothetical protein -
MUB19_RS06990 (MUB19_06965) 1361991..1362395 + 405 WP_003570045.1 MerR family transcriptional regulator -
MUB19_RS06995 (MUB19_06970) 1362388..1362714 + 327 WP_003584272.1 YrdB family protein -
MUB19_RS07000 (MUB19_06975) 1362777..1363406 + 630 WP_003570047.1 DUF2399 domain-containing protein -
MUB19_RS07005 (MUB19_06980) 1363451..1364104 + 654 WP_003570049.1 N-acetyltransferase -
MUB19_RS07010 (MUB19_06985) 1364478..1364747 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MUB19_RS07015 (MUB19_06990) 1364740..1365042 + 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
MUB19_RS07020 (MUB19_06995) 1365381..1365524 + 144 WP_162259784.1 hypothetical protein -
MUB19_RS07025 (MUB19_07000) 1365642..1366334 + 693 WP_003570055.1 hypothetical protein -
MUB19_RS07030 (MUB19_07005) 1366570..1368747 + 2178 WP_003570057.1 Tex family protein -
MUB19_RS07035 (MUB19_07010) 1369172..1369729 - 558 WP_003584278.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T240846 WP_016363448.1 NZ_CP094946:1364740-1365042 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T240846 NZ_LT838196:723945-724241 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT240846 WP_003570051.1 NZ_CP094946:1364478-1364747 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT240846 NZ_LT838196:724243-724638 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References