Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/Tad-couple_hipB
Location 668264..668912 Replicon chromosome
Accession NZ_CP094945
Organism Streptococcus thermophilus strain VHProbi R08

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag MUB28_RS03550 Protein ID WP_014727406.1
Coordinates 668264..668629 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID J7TXL7
Locus tag MUB28_RS03555 Protein ID WP_002890617.1
Coordinates 668619..668912 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUB28_RS03535 (MUB28_03535) 664771..665916 + 1146 WP_014727403.1 AbiH family protein -
MUB28_RS03540 (MUB28_03540) 666078..666797 + 720 WP_014727404.1 hypothetical protein -
MUB28_RS03545 (MUB28_03545) 666849..668099 + 1251 WP_014727405.1 restriction endonuclease subunit S -
MUB28_RS03550 (MUB28_03550) 668264..668629 + 366 WP_014727406.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MUB28_RS03555 (MUB28_03555) 668619..668912 + 294 WP_002890617.1 helix-turn-helix transcriptional regulator Antitoxin
MUB28_RS03560 (MUB28_03560) 668934..670535 + 1602 WP_041829288.1 type I restriction-modification system subunit M -
MUB28_RS03565 (MUB28_03565) 670613..671032 + 420 Protein_659 PD-(D/E)XK nuclease family transposase -
MUB28_RS09590 671133..672133 + 1001 Protein_660 DNA/RNA non-specific endonuclease -
MUB28_RS03580 (MUB28_03580) 672283..672843 + 561 WP_014621411.1 LemA family protein -
MUB28_RS03585 (MUB28_03585) 672845..673744 + 900 WP_014621412.1 zinc metalloprotease HtpX -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 660731..668912 8181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(36-109)

Antitoxin

(36-88)


Sequences


Toxin        


Download         Length: 122 a.a.        Molecular weight: 14511.65 Da        Isoelectric Point: 9.3397

>T240845 WP_014727406.1 NZ_CP094945:668264-668629 [Streptococcus thermophilus]
VHAIYFYKDKQGNQPVLDYMRELARHDSKDSRIKLNKLNDYIELLSQHGTCAGQPYIKHLDAEIWELRPLRDRILFVAWL
DGSFVLLHHFVKKTQKTPRREIEKAKRELQDLKERGLRDEE

Download         Length: 366 bp

>T240845 NZ_LT838196:658135-658449 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10778.52 Da        Isoelectric Point: 5.8962

>AT240845 WP_002890617.1 NZ_CP094945:668619-668912 [Streptococcus thermophilus]
MKNSAIGSNWKDVRAELFTKEEILESDLRVAIMTELIEARHEKGFSQKKLEELSGVSQPVIARMETGKTSPQLDTVLKVL
ASLGKTLAVVPLEQEKL

Download         Length: 294 bp

>AT240845 NZ_LT838196:658446-658862 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGCTCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB J7TXL7

References