Detailed information of TA system TA240843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4030547..4031241
• Replicon: chromosome
• Accession: NZ_CP094943
• Organism: Dickeya dadantii strain FZ06

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: MUB29_RS17720
• Protein ID: WP_263066119.1
• Coordinates: 4030547..4030945 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: G7LPQ3
• Locus tag: MUB29_RS17725
• Protein ID: WP_009111694.1
• Coordinates: 4030948..4031241 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUB29_RS17685	4026905..4027003	+	99	Protein_3462	SDR family NAD(P)-dependent oxidoreductase	-
MUB29_RS17690	4027049..4027240	-	192	Protein_3463	IS3 family transposase	-
MUB29_RS17695	4027514..4028743	-	1230	WP_226054963.1	GIY-YIG nuclease family protein	-
MUB29_RS17700	4028834..4029180	-	347	Protein_3465	hypothetical protein	-
MUB29_RS17705	4029207..4029398	+	192	Protein_3466	TerL protein	-
MUB29_RS17710	4029384..4029791	+	408	Protein_3467	phage minor head protein	-
MUB29_RS17715	4030112..4030249	+	138	Protein_3468	DUF2213 domain-containing protein	-
MUB29_RS17720	4030547..4030945	-	399	WP_263066119.1	type II toxin-antitoxin system YafO family toxin	Toxin
MUB29_RS17725	4030948..4031241	-	294	WP_009111694.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MUB29_RS17730	4031500..4032405	+	906	WP_263063509.1	hypothetical protein	-
MUB29_RS17735	4032395..4033243	+	849	WP_263063510.1	hypothetical protein	-
MUB29_RS17740	4033261..4033605	+	345	WP_263063511.1	hypothetical protein	-
MUB29_RS17745	4033746..4034483	+	738	WP_263063512.1	DUF2157 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15311.61 Da
• Isoelectric Point: 7.5155

>T240843 WP_263066119.1 NZ_CP094943:c4030945-4030547 [Dickeya dadantii]
IRIFKSALIRQQLSQQELDDLAADFLSYKKDGVLPDTFGRDAPYDDDRTYPLVKEEQVAHIHLADVNAPFPKFLRQFKRT
SDQAHLVYCQGAMDPDSYLLIIILKPEAHKMARNNNHMHKIGMMAEAFRMKH

Nucleotide

• Length: 399 bp

>T240843 NZ_LT827011:4584116-4584289 [Escherichia coli O127:H6]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10993.46 Da
• Isoelectric Point: 4.9167

>AT240843 WP_009111694.1 NZ_CP094943:c4031241-4030948 [Dickeya dadantii]
MERILAEKSINITELRKNPAKYFIDEPVAVLSNNRPAGYMVSAKVFEELIDLLEEKQGRVHTAARFRPTAERLSDIADSG
QKLLQNASDKDLTEFTE

Nucleotide

• Length: 294 bp

>AT240843 NZ_LT827011:4583792-4584088 [Escherichia coli O127:H6]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A2U1UKC7