Detailed information of TA system TA024073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 737823..738516
• Replicon: chromosome
• Accession: NC_012947
• Organism: Escherichia coli BL21-Gold(DE3)pLysS AG

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: ECBD_RS03690
• Protein ID: WP_000415584.1
• Coordinates: 737823..738119 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: ECBD_RS03695
• Protein ID: WP_000650107.1
• Coordinates: 738121..738516 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECBD_RS03655	732911..733225	-	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-
ECBD_RS03660	733256..733837	-	582	WP_000072420.1	NADPH:quinone oxidoreductase MdaB	-
ECBD_RS03665	734156..734488	+	333	WP_000917684.1	DUF2645 family protein	-
ECBD_RS03670	734534..735883	-	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
ECBD_RS03675	735880..736539	-	660	WP_001221493.1	two-component system response regulator QseB	-
ECBD_RS03680	736691..737083	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
ECBD_RS03685	737136..737618	+	483	WP_000183505.1	transcriptional regulator	-
ECBD_RS03690	737823..738119	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
ECBD_RS03695	738121..738516	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
ECBD_RS03700	738649..740256	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
ECBD_RS03705	740394..742652	+	2259	WP_001281877.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T24073 WP_000415584.1 NC_012947:737823-738119 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T24073 NC_012947:737823-738119 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT24073 WP_000650107.1 NC_012947:738121-738516 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT24073 NC_012947:738121-738516 [Escherichia coli 'BL21-Gold(DE3)pLysS AG']
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65