Detailed information of TA system TA024067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3551197..3551867
• Replicon: chromosome
• Accession: NC_012943
• Organism: Mycobacterium tuberculosis KZN 1435

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: TBMG_RS16805
• Protein ID: WP_003899954.1
• Coordinates: 3551197..3551541 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: G0THF6
• Locus tag: TBMG_RS16810
• Protein ID: WP_003899955.1
• Coordinates: 3551538..3551867 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TBMG_RS16775	3546270..3547130	+	861	WP_003416628.1	alpha/beta hydrolase	-
TBMG_RS16780	3547105..3547620	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
TBMG_RS16785	3547860..3548153	+	294	WP_003416635.1	hypothetical protein	-
TBMG_RS16790	3548441..3549730	+	1290	WP_003906996.1	ATP-binding protein	-
TBMG_RS16795	3550077..3550511	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
TBMG_RS16800	3550514..3550966	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
TBMG_RS16805	3551197..3551541	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
TBMG_RS16810	3551538..3551867	+	330	WP_003899955.1	XRE family transcriptional regulator	Antitoxin
TBMG_RS16815	3552405..3552752	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
TBMG_RS16820	3552749..3553369	+	621	WP_003899957.1	RES family NAD+ phosphorylase	-
TBMG_RS16825	3553529..3554794	-	1266	WP_003902423.1	hypothetical protein	-
TBMG_RS16830	3554962..3555171	+	210	WP_003416778.1	hypothetical protein	-
TBMG_RS16840	3555418..3556452	-	1035	WP_003416786.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T24067 WP_003899954.1 NC_012943:3551197-3551541 [Mycobacterium tuberculosis KZN 1435]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T24067 NC_012943:3551197-3551541 [Mycobacterium tuberculosis KZN 1435]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11802.46 Da
• Isoelectric Point: 7.4051

>AT24067 WP_003899955.1 NC_012943:3551538-3551867 [Mycobacterium tuberculosis KZN 1435]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Nucleotide

• Length: 330 bp

>AT24067 NC_012943:3551538-3551867 [Mycobacterium tuberculosis KZN 1435]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2

Antitoxin

• AlphaFold DB: G0THF6