Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3551197..3551867 Replicon chromosome
Accession NC_012943
Organism Mycobacterium tuberculosis KZN 1435

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag TBMG_RS16805 Protein ID WP_003899954.1
Coordinates 3551197..3551541 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag TBMG_RS16810 Protein ID WP_003899955.1
Coordinates 3551538..3551867 (+) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TBMG_RS16775 3546270..3547130 + 861 WP_003416628.1 alpha/beta hydrolase -
TBMG_RS16780 3547105..3547620 + 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
TBMG_RS16785 3547860..3548153 + 294 WP_003416635.1 hypothetical protein -
TBMG_RS16790 3548441..3549730 + 1290 WP_003906996.1 ATP-binding protein -
TBMG_RS16795 3550077..3550511 - 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
TBMG_RS16800 3550514..3550966 - 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
TBMG_RS16805 3551197..3551541 + 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
TBMG_RS16810 3551538..3551867 + 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
TBMG_RS16815 3552405..3552752 + 348 WP_003899956.1 DUF2384 domain-containing protein -
TBMG_RS16820 3552749..3553369 + 621 WP_003899957.1 RES family NAD+ phosphorylase -
TBMG_RS16825 3553529..3554794 - 1266 WP_003902423.1 hypothetical protein -
TBMG_RS16830 3554962..3555171 + 210 WP_003416778.1 hypothetical protein -
TBMG_RS16840 3555418..3556452 - 1035 WP_003416786.1 IS30 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T24067 WP_003899954.1 NC_012943:3551197-3551541 [Mycobacterium tuberculosis KZN 1435]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

>T24067 NC_012943:3551197-3551541 [Mycobacterium tuberculosis KZN 1435]
TTGGCCGTGATCCTGCTCCCGCAGGTCGAACGGTGGTTCTTCGCGCTCAACAGGGATGCGATGGCCTCGGTCACCGGCGC
CATCGACCTGCTCGAAATGGAGGGGCCGACGTTGGGCCGCCCGGTGGTCGACAAAGTGAACGACTCAACGTTTCACAACA
TGAAGGAGCTGCGCCCCGCCGGCACCAGCATCCGGATCCTGTTCGCCTTCGACCCGGCCCGGCAGGCGATCCTGCTGCTG
GGCGGTGACAAGGCAGGCAACTGGAAACGCTGGTACGACAACAACATTCCAATCGCTGACCAGCGCTCCGAGAACTGGCT
GGCGAGCGAGCACGGAGGTGGATGA

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT24067 WP_003899955.1 NC_012943:3551538-3551867 [Mycobacterium tuberculosis KZN 1435]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp

>AT24067 NC_012943:3551538-3551867 [Mycobacterium tuberculosis KZN 1435]
ATGACCATGGCCCGCAACTGGCGTGACATTCGCGCCGATGCCGTCGCGCAGGGCCGCGTGGATCTGCAGCGGGCCGCCGT
GGCACGCGAGGAGATGCGCGATGCCGTCCTGGCGCACCGCCTGGCCGAGATCCGCAAGGCGCTAGGCCACGCACGTCAGG
CCGACGTCGCGGCGCTGATGGGGGTCTCTCAGGCCCGTGTCTCCAAGCTGGAGAGCGGCGACCTGTCCCACACCGAACTC
GGCACCCTGCAGGCCTACGTTGCCGCCCTGGGCGGGCACCTGCGCATCGTCGCTGAGTTCGGCGAAAATACTGTCGAGCT
GACCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References