Detailed information of TA system TA240655

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5316838..5317433
• Replicon: chromosome
• Accession: NZ_CP094851
• Organism: Pseudomonas aeruginosa strain R20-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MUG61_RS24495
• Protein ID: WP_003117425.1
• Coordinates: 5317155..5317433 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MUG61_RS24490
• Protein ID: WP_003099268.1
• Coordinates: 5316838..5317143 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUG61_RS24460 (MUG61_24455)	5311957..5312244	-	288	WP_073651895.1	DUF5447 family protein	-
MUG61_RS24465 (MUG61_24460)	5312456..5312728	-	273	WP_003115921.1	hypothetical protein	-
MUG61_RS24470 (MUG61_24465)	5312838..5313104	+	267	WP_023088595.1	hypothetical protein	-
MUG61_RS24475 (MUG61_24470)	5313160..5313498	+	339	WP_073651894.1	hypothetical protein	-
MUG61_RS24480 (MUG61_24475)	5313591..5316086	+	2496	WP_134240666.1	hypothetical protein	-
MUG61_RS24485 (MUG61_24480)	5316128..5316436	-	309	WP_126475988.1	hypothetical protein	-
MUG61_RS24490 (MUG61_24485)	5316838..5317143	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MUG61_RS24495 (MUG61_24490)	5317155..5317433	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUG61_RS24500 (MUG61_24495)	5317486..5317614	-	129	Protein_4838	integrase	-
MUG61_RS24505 (MUG61_24500)	5317762..5319990	+	2229	WP_003113525.1	TonB-dependent receptor	-
MUG61_RS24510 (MUG61_24505)	5320060..5320707	-	648	WP_003095021.1	carbonate dehydratase	-
MUG61_RS24515 (MUG61_24510)	5320769..5322007	-	1239	WP_019681675.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T240655 WP_003117425.1 NZ_CP094851:c5317433-5317155 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T240655 NZ_LT606949:c1068476-1068063 [Polynucleobacter necessarius]
GTGCTGAAGTACTTATTAGACACAAATATTGTTATTTATGTCATAAAGCGAAAACCACTAGAGGCGCTTAAAGTTTTCAA
CAAAAATGCTAATCGCATGGCTATATCAGCAATTACATTGGCAGAACTGATTTATGACGCTGAAAAAAGTGCGCATGTAG
ACAAAAATCTCAATGTTATTGAAGACTTTGTTAGCCATCTAGAGGGTTTGCCATATGACATTAATGCAACTCAGCATTAT
GGGCAAATTAAGGCTTTTCTAGAGCGCGCGGGTCAGCCCATTGGAATAAATGATATTCATATTGCAGCTCACGCCAGAAG
TCACGGCTTAACTTTGGTTACTAATAATATGTCTGAGTTTAAGAGGGTTCTAAACCTTGCTCTTGAAAACTGGGTTGCAG
CATCGCTCCCCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT240655 WP_003099268.1 NZ_CP094851:c5317143-5316838 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT240655 NZ_LT606949:c1068709-1068476 [Polynucleobacter necessarius]
TTGGCTATTTCAACAGTGTTCACCAACAACCGTACTCAGGCGGTGAGATTGCCTGCAGAAATGCGATTTCCGGAAAATGT
GCGCAAGGTTAACGTGCGCTCAAAAGGTCGTGAGCGCATCATTGCGCCTATAGAAAATATGTGGGACAACTTCTTTTTGA
CTGCAGAAGGTGTAAGCGAAGACTTCATGAATAAGAGAGGCTCTCAGGAGCAAGGCGATAGGAAACCTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5