Detailed information of TA system TA240654

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4662510..4663191
• Replicon: chromosome
• Accession: NZ_CP094851
• Organism: Pseudomonas aeruginosa strain R20-14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MUG61_RS21480
• Protein ID: WP_015503432.1
• Coordinates: 4662826..4663191 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MUG61_RS21475
• Protein ID: WP_016561576.1
• Coordinates: 4662510..4662833 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUG61_RS21440 (MUG61_21435)	4658402..4659085	-	684	WP_003120766.1	TetR/AcrR family transcriptional regulator	-
MUG61_RS21445 (MUG61_21440)	4659173..4660051	+	879	WP_003163191.1	hypothetical protein	-
MUG61_RS21455 (MUG61_21450)	4660654..4660881	+	228	WP_014603215.1	hypothetical protein	-
MUG61_RS21460 (MUG61_21455)	4660896..4661888	-	993	Protein_4244	tyrosine-type recombinase/integrase	-
MUG61_RS21465 (MUG61_21460)	4661888..4662280	-	393	Protein_4245	hypothetical protein	-
MUG61_RS21470 (MUG61_21465)	4662275..4662394	+	120	Protein_4246	IS5/IS1182 family transposase	-
MUG61_RS21475 (MUG61_21470)	4662510..4662833	-	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
MUG61_RS21480 (MUG61_21475)	4662826..4663191	-	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MUG61_RS21485 (MUG61_21480)	4663455..4663697	-	243	WP_043884955.1	hypothetical protein	-
MUG61_RS21490 (MUG61_21485)	4663904..4664176	+	273	WP_003085667.1	hypothetical protein	-
MUG61_RS21495 (MUG61_21490)	4664195..4664620	-	426	WP_003114206.1	VOC family protein	-
MUG61_RS21500 (MUG61_21495)	4664721..4665605	+	885	WP_016253805.1	LysR substrate-binding domain-containing protein	-
MUG61_RS21505 (MUG61_21500)	4665578..4666531	-	954	WP_003085661.1	LysR substrate-binding domain-containing protein	-
MUG61_RS21510 (MUG61_21505)	4666752..4667186	+	435	WP_003116494.1	RidA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T240654 WP_015503432.1 NZ_CP094851:c4663191-4662826 [Pseudomonas aeruginosa]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T240654 NZ_LT606949:c48015-47821 [Polynucleobacter necessarius]
ATGAATAGCAAAGAACTCATCAAAGCATTGATAAAAGATGGTTGGGTGTTGCGGAGCTCAAAAGGCTCTCATCATGTGTT
TAATCATCTCTGCAAAGGAGGTCATATAACCATTCCACACCCCAAAAAGGATGTGGGCATTGGAGTAGTTCAAAAACTAC
TCAAACAAGCTGACATTAAATCAGGAATCTTATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT240654 WP_016561576.1 NZ_CP094851:c4662833-4662510 [Pseudomonas aeruginosa]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT240654 NZ_LT606949:c47824-47432 [Polynucleobacter necessarius]
ATGAAATATCCAATTGCTATCGAACCTGGCAGTGATAAGACTGCATGGGGCGTTATTGTTCCTGATTTGCCAGGATGCTT
TTCTGCTGCCGACAGTGGAATTGATGAAGCCATTGAAAATGCCAAGGAAGCAATCGAACTTTGGATTGAAACGGCACTAG
ATGCAAGTCAAGACATCCCAAAACCCAGCTCAATTACTAGCTTACAAAAGAAAAAAGAATTTAGAGGATATGTCTGGGCT
ATTGCAGAGATTGATCCTGCTTTATTGTCAGACGACATTGAAAGAGTAAATATCTCATTACCCAAAAGGGTATTAGCTAG
ATTAGATGCGAAGGCTAAGTCCGCTGGTGAAAATCGCTCAGCCTTTATCGCTCATATGGCGATTAGCACTTAA