Detailed information of TA system TA240312

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF1/-
• Location: 371694..372215
• Replicon: chromosome
• Accession: NZ_CP094773
• Organism: Staphylococcus aureus strain IVB6195

Toxin (Protein)

• Gene name: holin
• Uniprot ID: A0A2S6DEP6
• Locus tag: MUA47_RS01675
• Protein ID: WP_000339141.1
• Coordinates: 371913..372215 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	371694..371833 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA47_RS01635 (366800)	366800..367090	+	291	WP_000179864.1	hypothetical protein	-
MUA47_RS01640 (367106)	367106..369016	+	1911	WP_250406127.1	hypothetical protein	-
MUA47_RS01645 (369016)	369016..370482	+	1467	WP_250425823.1	BppU family phage baseplate upper protein	-
MUA47_RS01650 (370482)	370482..370871	+	390	WP_250406125.1	DUF2977 domain-containing protein	-
MUA47_RS01655 (370864)	370864..371028	+	165	WP_000916021.1	XkdX family protein	-
MUA47_RS01660 (371074)	371074..371373	+	300	WP_000466774.1	DUF2951 family protein	-
MUA47_RS01665 (371526)	371526..371702	+	177	WP_072357969.1	type I toxin-antitoxin system toxin PepG1	-
- (371694)	371694..371833	-	140	NuclAT_0	-	Antitoxin
- (371694)	371694..371833	-	140	NuclAT_0	-	Antitoxin
- (371694)	371694..371833	-	140	NuclAT_0	-	Antitoxin
- (371694)	371694..371833	-	140	NuclAT_0	-	Antitoxin
MUA47_RS01670 (371755)	371755..371862	-	108	WP_042900009.1	hypothetical protein	-
MUA47_RS01675 (371913)	371913..372215	+	303	WP_000339141.1	phage holin	Toxin
MUA47_RS01680 (372226)	372226..373680	+	1455	WP_250425827.1	N-acetylmuramoyl-L-alanine amidase	-
MUA47_RS01685 (374776)	374776..375162	+	387	WP_228480554.1	hypothetical protein	-
MUA47_RS01690 (375722)	375722..376549	-	828	WP_262634724.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	329007..373680	44673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (1-71)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11174.92 Da
• Isoelectric Point: 9.6413

>T240312 WP_000339141.1 NZ_CP094773:371913-372215 [Staphylococcus aureus]
MDAKVITRYIVLILALVNQFLANKGISPIPVDDETISSIILTVVALYTTYKDNPTSQEGKWANQKLKKYKAENKYRKATG
QAPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T240312 NZ_LS992190:c4315491-4315204 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 140 bp

>AT240312 NZ_CP094773:c371833-371694 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGTGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• AlphaFold DB: A0A2S6DEP6

Antitoxin