Detailed information of TA system TA240212

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): holin-SprF3/-
• Location: 357640..358085
• Replicon: chromosome
• Accession: NZ_CP094758
• Organism: Staphylococcus aureus strain IVB6239

Toxin (Protein)

• Gene name: holin
• Uniprot ID: -
• Locus tag: MUA67_RS01670
• Protein ID: WP_262610945.1
• Coordinates: 357783..358085 (+)
• Length: 101 a.a.

Antitoxin (RNA)

Gene name	SprF3
Locus tag	-
Coordinates	357640..357695 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUA67_RS01630	352670..352960	+	291	WP_000179860.1	hypothetical protein	-
MUA67_RS01635	352976..354886	+	1911	WP_262610939.1	hypothetical protein	-
MUA67_RS01640	354886..356352	+	1467	WP_262610941.1	BppU family phage baseplate upper protein	-
MUA67_RS01645	356352..356741	+	390	WP_262610943.1	DUF2977 domain-containing protein	-
MUA67_RS01650	356734..356898	+	165	WP_000916021.1	XkdX family protein	-
MUA67_RS01655	356944..357243	+	300	WP_000466774.1	DUF2951 family protein	-
MUA67_RS01660	357396..357554	+	159	WP_262611649.1	putative holin-like toxin	-
-	357564..357703	-	140	NuclAT_0	-	-
-	357564..357703	-	140	NuclAT_0	-	-
-	357564..357703	-	140	NuclAT_0	-	-
-	357564..357703	-	140	NuclAT_0	-	-
MUA67_RS01665	357625..357732	-	108	WP_042900009.1	hypothetical protein	-
-	357640..357695	-	56	-	-	Antitoxin
MUA67_RS01670	357783..358085	+	303	WP_262610945.1	phage holin	Toxin
MUA67_RS01675	358096..359549	+	1454	Protein_332	N-acetylmuramoyl-L-alanine amidase	-
MUA67_RS01680	360645..361031	+	387	WP_228480554.1	hypothetical protein	-
MUA67_RS01685	361161..361349	+	189	WP_262610947.1	hypothetical protein	-
MUA67_RS01690	361591..362418	-	828	WP_000136014.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	314365..359549	45184

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Holin (1-68)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11202.98 Da
• Isoelectric Point: 9.6413

>T240212 WP_262610945.1 NZ_CP094758:357783-358085 [Staphylococcus aureus]
MDAKVITRYIVLILALVNQFLANKGISPIPVDDETISSIILTVVALYTTYKDNPTSQEGKWANQKLKKYKAENKYRKATG
QVPIKEVMTPTNMNDTNDLG

Nucleotide

• Length: 303 bp

>T240212 NZ_LS992180:c664886-664422 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Nucleotide

• Length: 56 bp

>AT240212 NZ_CP094758:c357695-357640 [Staphylococcus aureus]
AAAAAGGGCAACATGTGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Structures

Antitoxin