Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324576..325162 Replicon chromosome
Accession NZ_CP094512
Organism Klebsiella pneumoniae strain SHX180

Toxin (Protein)


Gene name doc Uniprot ID W9BMV7
Locus tag MTX44_RS01505 Protein ID WP_004185990.1
Coordinates 324794..325162 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MTX44_RS01500 Protein ID WP_004174006.1
Coordinates 324576..324797 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MTX44_RS01480 (MTX44_01480) 320733..321659 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MTX44_RS01485 (MTX44_01485) 321656..322933 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MTX44_RS01490 (MTX44_01490) 322930..323697 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MTX44_RS01495 (MTX44_01495) 323699..324412 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MTX44_RS01500 (MTX44_01500) 324576..324797 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MTX44_RS01505 (MTX44_01505) 324794..325162 + 369 WP_004185990.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MTX44_RS01510 (MTX44_01510) 325435..326751 + 1317 WP_004174008.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MTX44_RS01515 (MTX44_01515) 326858..327745 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MTX44_RS01520 (MTX44_01520) 327742..328587 + 846 WP_064158928.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MTX44_RS01525 (MTX44_01525) 328589..329659 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321656..330396 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(1-121)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13469.85 Da        Isoelectric Point: 8.0667

>T239906 WP_004185990.1 NZ_CP094512:324794-325162 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVCRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T239906 NZ_LS483391:1631075-1631410 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT239906 WP_004174006.1 NZ_CP094512:324576-324797 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT239906 NZ_LS483391:1630798-1631085 [Streptococcus pyogenes]
ATGACTACAGTAAAAAAAAACAGATCGGTTACCTTTCAAGCTAATAAAGAATTGGTAAGCGAAGCAATGACAGTATTAAA
CAAAAAAAATTTAACCTTATCATCTGCTTTAAGATTATTCCTACAAAATGTCGTTGTCACAAACGAGGTTGACTTATTGA
CGGAAGAGGAGTTAGAGAAAGAAAAACTTTTCAAGCAATTTCAAGCTGAAATCAACAAAAATATTGAGGATGTTCGTCAA
GGGAAGTTTTATACCTCTGAAGAAGTGAGGGCTGAACTTGGACTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BMV7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References