Detailed information of TA system TA239853

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 81159..81412
• Replicon: plasmid pKP4831-2
• Accession: NZ_CP094453
• Organism: Klebsiella pneumoniae subsp. rhinoscleromatis strain KP4831

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MRR30_RS29680
• Protein ID: WP_001312851.1
• Coordinates: 81159..81308 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	81353..81412 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MRR30_RS29640 (76518)	76518..76933	-	416	Protein_97	IS1-like element IS1B family transposase	-
MRR30_RS29645 (77182)	77182..77583	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
MRR30_RS29650 (77516)	77516..77773	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
MRR30_RS29655 (77866)	77866..78519	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
MRR30_RS29660 (79458)	79458..80315	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
MRR30_RS30155 (80308)	80308..80382	-	75	WP_001375168.1	RepA leader peptide Tap	-
MRR30_RS29675 (80627)	80627..80875	-	249	WP_000083837.1	replication regulatory protein RepA	-
MRR30_RS29680 (81159)	81159..81308	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (81353)	81353..81412	+	60	NuclAT_1	-	Antitoxin
- (81353)	81353..81412	+	60	NuclAT_1	-	Antitoxin
- (81353)	81353..81412	+	60	NuclAT_1	-	Antitoxin
- (81353)	81353..81412	+	60	NuclAT_1	-	Antitoxin
MRR30_RS29685 (81613)	81613..81945	-	333	WP_152916585.1	hypothetical protein	-
MRR30_RS29690 (82007)	82007..82606	-	600	WP_032083981.1	PIN domain-containing protein	-
MRR30_RS29695 (82992)	82992..83192	-	201	WP_015059022.1	hypothetical protein	-
MRR30_RS29700 (83324)	83324..83884	-	561	WP_000139328.1	fertility inhibition protein FinO	-
MRR30_RS29705 (83939)	83939..84685	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
MRR30_RS29710 (84705)	84705..84905	-	201	WP_072354025.1	hypothetical protein	-
MRR30_RS29715 (84930)	84930..85634	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MRR30_RS29720 (85687)	85687..85752	+	66	Protein_112	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaSHV-12 / blaKPC-2 / rmtB / blaTEM-1B	-	1..96608	96608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T239853 WP_001312851.1 NZ_CP094453:c81308-81159 [Klebsiella pneumoniae subsp. rhinoscleromatis]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T239853 NZ_LS483359:1517130-1517465 [Streptococcus pyogenes]
TTGGACTATAAGAAATATCAGATTATCTATGCTCCTGATGTTTTAGAGAAGCTAAAAGAAATTCGTGATTATATTTCTCA
AAACTATTCCTCAACATCAGGTCAGCGTAAAATGGAGCAAATCATTAGCGACATAGAAAAACTCGAGGTTTTCCCAGAAG
TTGGTTTTGATGCCGATGAAAAATATGGTTCAAAAATCATCCATTATCACAGCACTAAAGGTTATACCTTAAGTAAAGAT
TATATTGTCCTATACCATATCGAGGGGGAAGAAAATAGGGTCGTTATTGATTACTTGCTTCCTACTCAAAGCGACTATAT
AAAGTTATTCAAATAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT239853 NZ_CP094453:81353-81412 [Klebsiella pneumoniae subsp. rhinoscleromatis]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin