Detailed information of TA system TA023985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1706844..1707503
• Replicon: chromosome
• Accession: NC_012917
• Organism: Pectobacterium carotovorum subsp. carotovorum PC1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C6DDP0
• Locus tag: PC1_RS07280
• Protein ID: WP_015839728.1
• Coordinates: 1706844..1707182 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PC1_RS07285
• Protein ID: WP_015839729.1
• Coordinates: 1707201..1707503 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PC1_RS07260	1703154..1704410	+	1257	WP_015839724.1	multidrug effflux MFS transporter	-
PC1_RS07265	1704455..1705294	-	840	WP_015839725.1	glutathione-dependent disulfide-bond oxidoreductase	-
PC1_RS07270	1705462..1706073	-	612	WP_015839726.1	NAD(P)-dependent oxidoreductase	-
PC1_RS07275	1706176..1706616	+	441	WP_015839727.1	helix-turn-helix transcriptional regulator	-
PC1_RS07280	1706844..1707182	+	339	WP_015839728.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PC1_RS07285	1707201..1707503	+	303	WP_015839729.1	XRE family transcriptional regulator	Antitoxin
PC1_RS07290	1707493..1708692	-	1200	WP_015839730.1	multidrug effflux MFS transporter	-
PC1_RS07295	1708862..1710097	-	1236	WP_015839731.1	MFS transporter	-
PC1_RS07300	1710164..1710619	+	456	WP_015839732.1	helix-turn-helix domain-containing protein	-
PC1_RS07305	1710820..1712256	+	1437	WP_015839733.1	family 1 glycosylhydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 13095.18 Da
• Isoelectric Point: 10.0651

>T23985 WP_015839728.1 NC_012917:1706844-1707182 [Pectobacterium carotovorum subsp. carotovorum PC1]
VWRIETTDAFDRWLSALNDMDRACVLAALIVLREKGPMLPRPYADTVKGSRYSNMKELRIQSRGMPIRAFFAFDTHRRGI
LLCAGNKSGNEKRFYEEMIPFADREFTNYLKK

Nucleotide

• Length: 339 bp

>T23985 NC_012917:1706844-1707182 [Pectobacterium carotovorum subsp. carotovorum PC1]
GTGTGGAGAATTGAAACGACGGACGCGTTTGATCGCTGGTTAAGCGCACTAAACGATATGGATCGAGCTTGTGTGCTTGC
AGCACTTATCGTACTACGGGAAAAGGGGCCCATGTTACCTCGACCTTATGCTGATACGGTCAAAGGTTCCCGCTATAGCA
ACATGAAAGAGCTGCGTATTCAGAGCCGAGGGATGCCGATACGGGCATTTTTTGCTTTCGACACACATCGTAGAGGGATC
CTGCTTTGTGCAGGTAATAAGTCTGGTAATGAAAAACGCTTTTACGAAGAGATGATCCCGTTTGCCGATCGAGAATTCAC
CAATTATCTGAAAAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11160.96 Da
• Isoelectric Point: 6.0769

>AT23985 WP_015839729.1 NC_012917:1707201-1707503 [Pectobacterium carotovorum subsp. carotovorum PC1]
MGRTLEQLLADEKPEVVIDAHALAADMLLNIHLAELRERVQKTQVDMAQTLGLKQPTVAVMEKPGRDLKLSTLKRYVEAT
GSKLHLDVELPDGSHYVFTL

Nucleotide

• Length: 303 bp

>AT23985 NC_012917:1707201-1707503 [Pectobacterium carotovorum subsp. carotovorum PC1]
ATGGGCAGAACGCTGGAACAGCTTCTTGCGGATGAAAAACCAGAGGTCGTCATTGATGCTCACGCTCTTGCGGCAGATAT
GTTGCTCAATATTCACCTCGCTGAACTGCGTGAGAGGGTGCAAAAAACGCAAGTAGACATGGCGCAGACGCTTGGATTAA
AACAGCCGACCGTGGCGGTAATGGAGAAACCCGGTCGCGATCTGAAACTTTCCACACTCAAACGCTATGTTGAGGCGACC
GGCAGTAAGCTGCATCTTGATGTTGAGCTGCCGGACGGCTCTCATTACGTGTTCACGCTGTAG

Structures

Toxin

• AlphaFold DB: C6DDP0