Detailed information of TA system TA023976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1812311..1812963
• Replicon: chromosome
• Accession: NC_012913
• Organism: Aggregatibacter aphrophilus NJ8700

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: NT05HA_RS08430
• Protein ID: WP_005702038.1
• Coordinates: 1812616..1812963 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: NT05HA_RS08425
• Protein ID: WP_005702039.1
• Coordinates: 1812311..1812619 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NT05HA_RS08400	1807913..1808602	-	690	WP_005702044.1	50S ribosomal protein L1	-
NT05HA_RS08405	1808607..1809035	-	429	WP_005630840.1	50S ribosomal protein L11	-
NT05HA_RS08410	1809205..1809756	-	552	WP_005702043.1	transcription termination/antitermination protein NusG	-
NT05HA_RS08415	1809758..1810168	-	411	WP_005702041.1	preprotein translocase subunit SecE	-
NT05HA_RS08420	1811033..1811548	+	516	WP_005702040.1	Hcp family type VI secretion system effector	-
NT05HA_RS08425	1812311..1812619	-	309	WP_005702039.1	helix-turn-helix domain-containing protein	Antitoxin
NT05HA_RS08430	1812616..1812963	-	348	WP_005702038.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NT05HA_RS08435	1813026..1813583	-	558	WP_005702037.1	hypothetical protein	-
NT05HA_RS08440	1813570..1813758	-	189	WP_005702035.1	hypothetical protein	-
NT05HA_RS08445	1813758..1814003	-	246	WP_005702034.1	hypothetical protein	-
NT05HA_RS08450	1813996..1814838	-	843	WP_049756048.1	host cell division inhibitor Icd-like protein	-
NT05HA_RS08455	1815078..1815380	-	303	WP_005702032.1	hypothetical protein	-
NT05HA_RS08460	1815660..1816157	-	498	WP_005702031.1	hypothetical protein	-
NT05HA_RS08465	1816229..1816994	-	766	Protein_1631	phage antirepressor Ant	-
NT05HA_RS08475	1817288..1817671	-	384	WP_005594660.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (13-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13326.24 Da
• Isoelectric Point: 9.7797

>T23976 WP_005702038.1 NC_012913:c1812963-1812616 [Aggregatibacter aphrophilus NJ8700]
VKLLFIELPPFERYRKVHLSDDEYRAFQNELLENPEKGDVIQNTGGLRKIRIADSERNKGKRGGARVIYYYFLDGAQIWL
FSAYGKGEKADLNESEKRAFKVVLENLKAMARGKE

Nucleotide

• Length: 348 bp

>T23976 NC_012913:c1812963-1812616 [Aggregatibacter aphrophilus NJ8700]
GTGAAACTATTATTTATTGAATTACCGCCCTTTGAGCGTTATAGAAAAGTGCATTTATCTGATGATGAATATCGGGCTTT
TCAAAATGAGTTATTAGAAAACCCCGAAAAAGGCGATGTGATACAAAATACAGGCGGATTGAGAAAAATTAGAATCGCTG
ACAGTGAGAGAAACAAAGGGAAACGTGGTGGCGCACGGGTGATTTATTACTACTTTTTAGACGGTGCGCAAATATGGCTT
TTTAGTGCTTACGGAAAAGGCGAAAAAGCAGACTTAAACGAAAGCGAGAAACGCGCATTTAAAGTGGTATTAGAGAATTT
AAAGGCAATGGCAAGGGGGAAAGAATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11508.24 Da
• Isoelectric Point: 9.8103

>AT23976 WP_005702039.1 NC_012913:c1812619-1812311 [Aggregatibacter aphrophilus NJ8700]
MSRLFDDLMEGAQALEQHLQGKITLRTETRERIEPASISAEEVKAIREKLNLSQAVFAQKLRTSVRTYQGWEQGKTKPSA
HASLLLRMVDKAPQTFELIAGI

Nucleotide

• Length: 309 bp

>AT23976 NC_012913:c1812619-1812311 [Aggregatibacter aphrophilus NJ8700]
ATGAGCCGTTTATTTGATGATTTAATGGAAGGCGCGCAGGCTCTTGAGCAACATTTACAAGGTAAAATCACACTACGCAC
AGAAACGCGCGAACGAATTGAACCCGCAAGCATTAGTGCGGAGGAAGTGAAAGCAATTAGAGAGAAATTAAACCTTTCTC
AAGCTGTTTTTGCGCAGAAGTTACGAACCAGTGTAAGAACCTATCAAGGTTGGGAGCAAGGCAAAACCAAACCTAGCGCG
CACGCCTCTTTATTGTTGCGCATGGTGGACAAAGCACCGCAAACCTTCGAGCTTATCGCAGGGATATAA