Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 3840379..3841001 Replicon chromosome
Accession NZ_CP094343
Organism Pseudomonas sp. M1

Toxin (Protein)


Gene name higB Uniprot ID W5IUR9
Locus tag PM1_RS16950 Protein ID WP_009619926.1
Coordinates 3840379..3840693 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W5IVT4
Locus tag PM1_RS16955 Protein ID WP_009619925.1
Coordinates 3840696..3841001 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PM1_RS16930 (MRY70_16930) 3835891..3836310 + 420 WP_009619934.1 LytTR family DNA-binding domain-containing protein -
PM1_RS16935 (MRY70_16935) 3836616..3837041 - 426 WP_009619932.1 hypothetical protein -
PM1_RS16940 (MRY70_16940) 3837473..3837823 + 351 WP_009619928.1 hypothetical protein -
PM1_RS16945 (MRY70_16945) 3837998..3838201 - 204 WP_024128400.1 hypothetical protein -
PM1_RS16950 (MRY70_16950) 3840379..3840693 + 315 WP_009619926.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PM1_RS16955 (MRY70_16955) 3840696..3841001 + 306 WP_009619925.1 helix-turn-helix domain-containing protein Antitoxin
PM1_RS16960 (MRY70_16960) 3841397..3841681 + 285 WP_009619924.1 hypothetical protein -
PM1_RS16965 (MRY70_16965) 3841865..3842251 + 387 Protein_3355 integrase -
PM1_RS16975 (MRY70_16975) 3842738..3843163 - 426 WP_009619921.1 lipocalin-like domain-containing protein -
PM1_RS16980 (MRY70_16980) 3843168..3844004 - 837 WP_009619920.1 alpha/beta fold hydrolase -
PM1_RS16985 (MRY70_16985) 3844001..3844960 - 960 WP_009619919.1 VOC family protein -
PM1_RS16990 (MRY70_16990) 3844995..3845741 - 747 WP_009619918.1 glucose 1-dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(36-72)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11774.51 Da        Isoelectric Point: 9.4385

>T239748 WP_009619926.1 NZ_CP094343:3840379-3840693 [Pseudomonas sp. M1]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGAVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLVY
DKTSQEDLTADQKKVLRQIVENWR

Download         Length: 315 bp

>T239748 NZ_LS483312:971885-972235 [Staphylococcus lugdunensis]
ATGATTAGACGCGGCGATGTATATTTAGCTGATTTATCACCGGTACAAGGGTCTGAACAAGGGGGAGTAAGACCAGTTGT
CATCATTCAAAATGACACCGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCAATTACTGGAAGGATTAATAAAGCGA
AAATACCCACTCATGTGGAAATAGGTAAAAAGAAATATAAGTTAGATAAAGATTCTGTTATTCTGTTAGAGCAAATACGA
ACATTAGATAAAAAAAGATTAAAAGAAAAACTTACTTATCTCTCTGAGGATAAAATGAAAGAAGTAGATCATGCCCTAGA
CATTAGTCTAGGTTTACATAACTATCATTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11385.90 Da        Isoelectric Point: 7.2080

>AT239748 WP_009619925.1 NZ_CP094343:3840696-3841001 [Pseudomonas sp. M1]
MDKELFAELVESVTQMDEIVRGERQPSREFHIDAVKVREIRRATGLSQAKFARKINVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRED

Download         Length: 306 bp

>AT239748 NZ_LS483312:971718-971888 [Staphylococcus lugdunensis]
ATGCTAACTTTTAATCAAAATCGTAGTCATAGTCTAGAACAGTCATTAAAAGAAGGGTATGCTCAGATGGCAGACCTTAA
TCTCTCCCTAGCAACAGAGGCTTTCCCAATTGAATGTGAAGCTTGTGATTGCAATGAAACTTATCTAACTTCCAATCATC
AGAGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W5IUR9

Antitoxin

Source ID Structure
AlphaFold DB A0A127MSV9

References