Detailed information of TA system TA239747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3811538..3812151
• Replicon: chromosome
• Accession: NZ_CP094343
• Organism: Pseudomonas sp. M1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: W5IX19
• Locus tag: PM1_RS16810
• Protein ID: WP_009619978.1
• Coordinates: 3811855..3812151 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PM1_RS16805
• Protein ID: WP_024128404.1
• Coordinates: 3811538..3811846 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PM1_RS16780 (MRY70_16780)	3806777..3807697	+	921	WP_009619989.1	bestrophin family ion channel	-
PM1_RS16785 (MRY70_16785)	3807714..3808280	+	567	WP_009619987.1	flavin reductase family protein	-
PM1_RS16790 (MRY70_16790)	3808467..3809846	-	1380	WP_009619986.1	ATP-binding protein	-
PM1_RS16795 (MRY70_16795)	3809824..3810513	-	690	WP_009619984.1	response regulator transcription factor	-
PM1_RS16800 (MRY70_16800)	3810618..3811325	+	708	WP_009619982.1	response regulator transcription factor	-
PM1_RS16805 (MRY70_16805)	3811538..3811846	-	309	WP_024128404.1	HigA family addiction module antitoxin	Antitoxin
PM1_RS16810 (MRY70_16810)	3811855..3812151	-	297	WP_009619978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PM1_RS16815 (MRY70_16815)	3812374..3813777	+	1404	WP_009619976.1	efflux transporter outer membrane subunit	-
PM1_RS16820 (MRY70_16820)	3813774..3814940	+	1167	WP_009619974.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11751.18 Da
• Isoelectric Point: 7.4023

>T239747 WP_009619978.1 NZ_CP094343:c3812151-3811855 [Pseudomonas sp. M1]
MIGSFRDAWLEAFFVRDRMPRQIPADIQERLFRKLQMLDDATTDADLRSPPSNHFERLQGSLRAYHSIRVNRQWRLVFHW
DGEKGEASDVYLDNHSYR

Nucleotide

• Length: 297 bp

>T239747 NZ_LS483312:960623-960835 [Staphylococcus lugdunensis]
GTGGAAAAGCGATTAATTTATATTACAAATGTGACTAGTGAAGACGACTTGAAAAACTTACGAACAAGGTTATTAGAGAT
GATTGGTGTTAATGAAGTAGTGATCAATTTTGATGAACGTGCAGTTAATGTTACTTTTGAAACACCAACTAATTTAAATG
CACTTGAAAAAGAAATATATGATGCTGGCTATCATGCTATAGATGATTTATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11471.20 Da
• Isoelectric Point: 11.1591

>AT239747 WP_024128404.1 NZ_CP094343:c3811846-3811538 [Pseudomonas sp. M1]
VIITERKPVSVGEVLSSEFLDPLGLTQGRLAEAMGVQRKLVNELCNDRRAVTADTALMLARVFGNSAEFWLNLQRRNDLW
AALNSPERRARIERARPLNRAA

Nucleotide

• Length: 309 bp

>AT239747 NZ_LS483312:960317-960610 [Staphylococcus lugdunensis]
TTGAACGAACAAGATCATGCACATCATTCTAAAATTGTAAAAGCAAATTTAAAATCTAGATTAAATCGTATAGAAGGACA
AGTGCGGGCAATTAATCGCATGGTTGAAGATGATGTTTATTGTGATGATGTGTTAACACAAATAAGAGCAACACGCTCAG
CATTAACGAGTGTGGCAACAAAACTATTAGAGCATCATATGAAAAGTTGTATCATGGATAAAATAAACGATGGGCAGGAA
GTTGAAGCGATGGAGGAATTGCTAGTTACATTTCAAAAAATGCTAAAAGACTAG

Structures

Toxin

• AlphaFold DB: W5IX19