Detailed information of TA system TA239741

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27022..27440
• Replicon: plasmid p2
• Accession: NZ_CP094341
• Organism: Escherichia coli strain W224N

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MQV61_RS25995
• Protein ID: WP_223412041.1
• Coordinates: 27318..27440 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27022..27226 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQV61_RS25960	22722..23243	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
MQV61_RS25965	23299..23532	+	234	WP_000005971.1	DUF905 domain-containing protein	-
MQV61_RS25970	23596..25554	+	1959	WP_012881137.1	ParB/RepB/Spo0J family partition protein	-
MQV61_RS25975	25609..26043	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
MQV61_RS25980	26040..26759	+	720	WP_242935526.1	plasmid SOS inhibition protein A	-
MQV61_RS25985	26756..27070	+	315	WP_000872086.1	hypothetical protein	-
-	27019..27226	+	208	NuclAT_0	-	-
-	27019..27226	+	208	NuclAT_0	-	-
-	27019..27226	+	208	NuclAT_0	-	-
-	27019..27226	+	208	NuclAT_0	-	-
-	27022..27226	-	205	-	-	Antitoxin
MQV61_RS25990	27260..27373	+	114	Protein_36	DUF5431 family protein	-
MQV61_RS25995	27318..27440	+	123	WP_223412041.1	Hok/Gef family protein	Toxin
MQV61_RS26000	27741..27990	-	250	Protein_38	hypothetical protein	-
MQV61_RS26005	28286..28609	+	324	WP_000533253.1	hypothetical protein	-
MQV61_RS26010	28668..28910	+	243	WP_000540588.1	hypothetical protein	-
MQV61_RS26015	29176..29436	+	261	WP_071600428.1	hypothetical protein	-
MQV61_RS26020	29907..30203	+	297	WP_001272251.1	hypothetical protein	-
MQV61_RS26025	30313..31134	+	822	WP_001234437.1	DUF932 domain-containing protein	-
MQV61_RS26030	31430..32020	-	591	WP_000252686.1	transglycosylase SLT domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..74517	74517

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4560.33 Da
• Isoelectric Point: 8.2774

>T239741 WP_223412041.1 NZ_CP094341:27318-27440 [Escherichia coli]
IVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T239741 NZ_LS483311:c2176099-2175737 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Nucleotide

• Length: 205 bp

>AT239741 NZ_CP094341:c27226-27022 [Escherichia coli]
AGACATACAGGGGGCCTCGTGGGTTAATGAAAAATTAACTACGGGGCTTTCTGCCTTCTGCCACACAACACGGTAACAAA
CCACCTTCACGTCATGAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCGCACTGACCACACTCAC
TTTCCCTGAAAATAATCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Antitoxin