Detailed information of TA system TA239735

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 70587..71013
• Replicon: plasmid p1
• Accession: NZ_CP094340
• Organism: Escherichia coli strain W224N

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MQV61_RS25650
• Protein ID: WP_001372321.1
• Coordinates: 70587..70712 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	70789..71013 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQV61_RS25610 (65961)	65961..66650	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
MQV61_RS25615 (66837)	66837..67220	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MQV61_RS25620 (67541)	67541..68143	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
MQV61_RS25625 (68440)	68440..69261	-	822	WP_001234426.1	DUF932 domain-containing protein	-
MQV61_RS25630 (69379)	69379..69666	-	288	WP_000107535.1	hypothetical protein	-
MQV61_RS25635 (69691)	69691..69897	-	207	WP_000547968.1	hypothetical protein	-
MQV61_RS25640 (69967)	69967..70139	+	173	Protein_75	hypothetical protein	-
MQV61_RS25645 (70137)	70137..70367	-	231	WP_071586998.1	hypothetical protein	-
MQV61_RS25650 (70587)	70587..70712	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
MQV61_RS25655 (70654)	70654..70803	-	150	Protein_78	plasmid maintenance protein Mok	-
- (70789)	70789..71013	-	225	NuclAT_0	-	Antitoxin
- (70789)	70789..71013	-	225	NuclAT_0	-	Antitoxin
- (70789)	70789..71013	-	225	NuclAT_0	-	Antitoxin
- (70789)	70789..71013	-	225	NuclAT_0	-	Antitoxin
MQV61_RS25660 (70825)	70825..71013	+	189	WP_001299721.1	hypothetical protein	-
MQV61_RS25665 (70982)	70982..71744	-	763	Protein_80	plasmid SOS inhibition protein A	-
MQV61_RS25670 (71741)	71741..72175	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
MQV61_RS25675 (72230)	72230..72427	-	198	Protein_82	hypothetical protein	-
MQV61_RS25680 (72455)	72455..72688	-	234	WP_000005987.1	DUF905 family protein	-
MQV61_RS25685 (72756)	72756..73295	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
MQV61_RS25690 (73321)	73321..73527	-	207	WP_000275856.1	hypothetical protein	-
MQV61_RS25695 (73597)	73597..73677	+	81	Protein_86	hypothetical protein	-
MQV61_RS25700 (73860)	73860..74029	-	170	Protein_87	hypothetical protein	-
MQV61_RS25705 (74666)	74666..75637	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T239735 WP_001372321.1 NZ_CP094340:c70712-70587 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T239735 NZ_LS483311:c317239-316778 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAATAAGAGAGGCTGATGTGCTTCCAGATAATTTGGA
TGGGGTATGGTTAGGAGATTTAATTTTAATAAAGCGTGGCTTATCAGATAGAGAAAAGGCAGGAATTCTCTTTGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCCGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTACGCAAGG
CGACACGGCTTTATCTCAGCAGTCCCGTTACGCGAAATTGTGGAAGCTTACAATTATGGTGTACGTAACTTGTATGAGTT
GTCTGAGTATCTGCAATTGAGTGAAGAATACATATTAGAAGCAATAGAACAATATAAAAAGATATATGGTATTGGGACTC
ACTACGGCGAATACTCAATTACATTTGAGCCATTGAGAGTTTTTAAATATAAAGAAATATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT239735 NZ_CP094340:c71013-70789 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin