Detailed information of TA system TA239734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4949064..4949666
• Replicon: chromosome
• Accession: NZ_CP094339
• Organism: Escherichia coli strain W224N

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: MQV61_RS24235
• Protein ID: WP_000897302.1
• Coordinates: 4949355..4949666 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MQV61_RS24230
• Protein ID: WP_000356397.1
• Coordinates: 4949064..4949354 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQV61_RS24205 (4945137)	4945137..4946039	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MQV61_RS24210 (4946036)	4946036..4946671	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MQV61_RS24215 (4946668)	4946668..4947597	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MQV61_RS24220 (4947813)	4947813..4948031	-	219	WP_001314326.1	CopG family transcriptional regulator	-
MQV61_RS24225 (4948427)	4948427..4948705	-	279	WP_001296612.1	hypothetical protein	-
MQV61_RS24230 (4949064)	4949064..4949354	-	291	WP_000356397.1	NadS family protein	Antitoxin
MQV61_RS24235 (4949355)	4949355..4949666	-	312	WP_000897302.1	hypothetical protein	Toxin
MQV61_RS24240 (4949895)	4949895..4950803	+	909	WP_001331553.1	alpha/beta hydrolase	-
MQV61_RS24245 (4950867)	4950867..4951808	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MQV61_RS24250 (4951853)	4951853..4952290	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MQV61_RS24255 (4952287)	4952287..4953159	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MQV61_RS24260 (4953153)	4953153..4953752	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T239734 WP_000897302.1 NZ_CP094339:c4949666-4949355 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T239734 NZ_LS483310:c2437139-2436897 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAGACATTTATAGAAATTGTTGAGACTTT
AAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATTTAGAGCGATATTCAAGAAGAATTA
ACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATACTATCGGCATGGTCACATTATGAT
TAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT239734 WP_000356397.1 NZ_CP094339:c4949354-4949064 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT239734 NZ_LS483310:c2437396-2437139 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: S1P416