Detailed information of TA system TA239728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3944088..3944782
• Replicon: chromosome
• Accession: NZ_CP094339
• Organism: Escherichia coli strain W224N

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MQV61_RS19510
• Protein ID: WP_001263500.1
• Coordinates: 3944088..3944486 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MQV61_RS19515
• Protein ID: WP_000554758.1
• Coordinates: 3944489..3944782 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQV61_RS19485 (3939453)	3939453..3939911	-	459	WP_001291988.1	xanthine phosphoribosyltransferase	-
MQV61_RS19490 (3940172)	3940172..3941629	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MQV61_RS19495 (3941686)	3941686..3942300	-	615	WP_000602123.1	peptide chain release factor H	-
MQV61_RS19500 (3942297)	3942297..3943436	-	1140	WP_000521577.1	RNA ligase RtcB family protein	-
MQV61_RS19505 (3943626)	3943626..3944078	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MQV61_RS19510 (3944088)	3944088..3944486	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MQV61_RS19515 (3944489)	3944489..3944782	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MQV61_RS19520 (3944834)	3944834..3945889	-	1056	WP_001226168.1	DNA polymerase IV	-
MQV61_RS19525 (3945960)	3945960..3946745	-	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
MQV61_RS19530 (3946717)	3946717..3948429	+	1713	Protein_3827	flagellar biosynthesis protein FlhA	-
MQV61_RS19535 (3948508)	3948508..3948666	+	159	WP_014639450.1	hypothetical protein	-
MQV61_RS19540 (3948749)	3948749..3949246	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3944088..3964442	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T239728 WP_001263500.1 NZ_CP094339:c3944486-3944088 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T239728 NZ_LS483310:c1831258-1831106 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT239728 WP_000554758.1 NZ_CP094339:c3944782-3944489 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT239728 NZ_LS483310:c1831882-1831283 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGACACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3