Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3712589..3713268 Replicon chromosome
Accession NZ_CP094339
Organism Escherichia coli strain W224N

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag MQV61_RS18440 Protein ID WP_000057524.1
Coordinates 3712966..3713268 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MQV61_RS18435 Protein ID WP_000806442.1
Coordinates 3712589..3712930 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQV61_RS18425 (3708833) 3708833..3709765 - 933 WP_000883052.1 glutaminase A -
MQV61_RS18430 (3710027) 3710027..3712531 + 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
MQV61_RS18435 (3712589) 3712589..3712930 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MQV61_RS18440 (3712966) 3712966..3713268 - 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MQV61_RS18445 (3713401) 3713401..3714195 + 795 WP_000365177.1 TraB/GumN family protein -
MQV61_RS18450 (3714399) 3714399..3714878 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MQV61_RS18455 (3714902) 3714902..3715702 + 801 WP_000439796.1 hypothetical protein -
MQV61_RS18460 (3715699) 3715699..3716202 + 504 WP_000667000.1 hypothetical protein -
MQV61_RS18465 (3716240) 3716240..3717892 - 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3705278..3716202 10924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T239726 WP_000057524.1 NZ_CP094339:c3713268-3712966 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T239726 NZ_LS483309:c2643792-2643526 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT239726 WP_000806442.1 NZ_CP094339:c3712930-3712589 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT239726 NZ_LS483309:c2644049-2643792 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References