Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2592415..2592977 Replicon chromosome
Accession NZ_CP094339
Organism Escherichia coli strain W224N

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MQV61_RS12645 Protein ID WP_000605675.1
Coordinates 2592415..2592693 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MQV61_RS12650 Protein ID WP_000781370.1
Coordinates 2592693..2592977 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQV61_RS12620 (2588002) 2588002..2588433 - 432 WP_000152310.1 peroxiredoxin OsmC -
MQV61_RS12625 (2588778) 2588778..2588993 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MQV61_RS12630 (2589095) 2589095..2589232 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MQV61_RS12635 (2589388) 2589388..2591085 + 1698 WP_000433462.1 malate dehydrogenase -
MQV61_RS12640 (2591219) 2591219..2592229 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
MQV61_RS12645 (2592415) 2592415..2592693 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MQV61_RS12650 (2592693) 2592693..2592977 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MQV61_RS12655 (2593206) 2593206..2593859 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MQV61_RS12660 (2593852) 2593852..2594736 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MQV61_RS12665 (2594749) 2594749..2597796 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T239721 WP_000605675.1 NZ_CP094339:2592415-2592693 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T239721 NZ_LS483309:c2240239-2239877 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT239721 WP_000781370.1 NZ_CP094339:2592693-2592977 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT239721 NZ_LS483309:c2240406-2240236 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References