Detailed information of TA system TA239361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4006114..4006711
• Replicon: chromosome
• Accession: NZ_CP094237
• Organism: Klebsiella pneumoniae strain K41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MQE26_RS19660
• Protein ID: WP_242898374.1
• Coordinates: 4006394..4006711 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: MQE26_RS19655
• Protein ID: WP_004142561.1
• Coordinates: 4006114..4006401 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE26_RS19625 (MQE26_19625)	4002194..4002442	+	249	WP_002893037.1	DUF1158 domain-containing protein	-
MQE26_RS19630 (MQE26_19630)	4002460..4002801	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
MQE26_RS19635 (MQE26_19635)	4002832..4003947	-	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
MQE26_RS19640 (MQE26_19640)	4004127..4004708	+	582	WP_004176968.1	TetR/AcrR family transcriptional regulator	-
MQE26_RS19645 (MQE26_19645)	4004708..4005076	+	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
MQE26_RS19650 (MQE26_19650)	4005196..4005849	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
MQE26_RS19655 (MQE26_19655)	4006114..4006401	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
MQE26_RS19660 (MQE26_19660)	4006394..4006711	-	318	WP_242898374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQE26_RS19665 (MQE26_19665)	4006896..4007939	-	1044	WP_023284821.1	DUF2157 domain-containing protein	-
MQE26_RS19670 (MQE26_19670)	4008606..4009472	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
MQE26_RS19675 (MQE26_19675)	4009581..4011008	+	1428	WP_102001330.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T239361 WP_242898374.1 NZ_CP094237:c4006711-4006394 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALRESDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T239361 NZ_LR890692:c201024-200737 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT239361 WP_004142561.1 NZ_CP094237:c4006401-4006114 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT239361 NZ_LR890692:c201263-201024 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1