Detailed information of TA system TA239358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 2502091..2502780
• Replicon: chromosome
• Accession: NZ_CP094237
• Organism: Klebsiella pneumoniae strain K41

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A331C6E2
• Locus tag: MQE26_RS12195
• Protein ID: WP_021469727.1
• Coordinates: 2502091..2502408 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MQE26_RS12200
• Protein ID: WP_040147778.1
• Coordinates: 2502484..2502780 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQE26_RS12165 (MQE26_12165)	2497793..2498302	+	510	WP_002906702.1	GNAT family N-acetyltransferase	-
MQE26_RS12170 (MQE26_12170)	2498312..2499238	+	927	WP_004189808.1	amino acid ABC transporter permease	-
MQE26_RS12175 (MQE26_12175)	2499222..2500001	+	780	WP_002906697.1	amino acid ABC transporter ATP-binding protein	-
MQE26_RS12180 (MQE26_12180)	2500040..2500891	+	852	WP_014343117.1	transporter substrate-binding domain-containing protein	-
MQE26_RS12185 (MQE26_12185)	2500969..2501586	+	618	WP_004175955.1	glutathione S-transferase family protein	-
MQE26_RS12190 (MQE26_12190)	2501657..2501884	+	228	WP_002906690.1	tautomerase PptA	-
MQE26_RS12195 (MQE26_12195)	2502091..2502408	+	318	WP_021469727.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQE26_RS12200 (MQE26_12200)	2502484..2502780	+	297	WP_040147778.1	NadS family protein	Antitoxin
MQE26_RS12205 (MQE26_12205)	2502859..2503305	+	447	WP_004175959.1	hypothetical protein	-
MQE26_RS12210 (MQE26_12210)	2503346..2504962	-	1617	WP_040147777.1	carbohydrate porin	-
MQE26_RS12215 (MQE26_12215)	2505006..2506388	-	1383	Protein_2391	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12221.10 Da
• Isoelectric Point: 10.2217

>T239358 WP_021469727.1 NZ_CP094237:2502091-2502408 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Nucleotide

• Length: 318 bp

>T239358 NZ_LR890691:c5103575-5103192 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11032.88 Da
• Isoelectric Point: 6.4721

>AT239358 WP_040147778.1 NZ_CP094237:2502484-2502780 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMPMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRQPTGAARK
LLLLLQGNPLIINLLKVV

Nucleotide

• Length: 297 bp

>AT239358 NZ_LR890691:c5103817-5103575 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A331C6E2