Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 321449..322035 Replicon chromosome
Accession NZ_CP094237
Organism Klebsiella pneumoniae strain K41

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MQE26_RS01495 Protein ID WP_002920800.1
Coordinates 321667..322035 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MQE26_RS01490 Protein ID WP_004174006.1
Coordinates 321449..321670 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MQE26_RS01470 (MQE26_01470) 317606..318532 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
MQE26_RS01475 (MQE26_01475) 318529..319806 + 1278 WP_004174005.1 branched chain amino acid ABC transporter permease LivM -
MQE26_RS01480 (MQE26_01480) 319803..320570 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MQE26_RS01485 (MQE26_01485) 320572..321285 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MQE26_RS01490 (MQE26_01490) 321449..321670 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MQE26_RS01495 (MQE26_01495) 321667..322035 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MQE26_RS01500 (MQE26_01500) 322308..323624 + 1317 WP_032435158.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MQE26_RS01505 (MQE26_01505) 323731..324618 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MQE26_RS01510 (MQE26_01510) 324615..325460 + 846 WP_004185988.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MQE26_RS01515 (MQE26_01515) 325462..326532 + 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 318529..327269 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T239344 WP_002920800.1 NZ_CP094237:321667-322035 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T239344 NZ_LR890686:c5206841-5206458 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT239344 WP_004174006.1 NZ_CP094237:321449-321670 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT239344 NZ_LR890686:c5207083-5206841 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References