Detailed information of TA system TA239308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1616738..1617326
• Replicon: chromosome
• Accession: NZ_CP094173
• Organism: Helicobacter pylori strain Hpfe0001

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K2LF18
• Locus tag: MPG49_RS07815
• Protein ID: WP_000921926.1
• Coordinates: 1617045..1617326 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: T5CU17
• Locus tag: MPG49_RS07810
• Protein ID: WP_000065285.1
• Coordinates: 1616738..1617052 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG49_RS07790 (MPG49_07790)	1612139..1613101	+	963	Protein_1518	type IV secretory system conjugative DNA transfer family protein	-
MPG49_RS07795 (MPG49_07795)	1613191..1613637	-	447	WP_162966965.1	IS200/IS605 family transposase	-
MPG49_RS07800 (MPG49_07800)	1613689..1614972	+	1284	WP_245052296.1	transposase	-
MPG49_RS07805 (MPG49_07805)	1614969..1616444	-	1476	WP_245052297.1	relaxase/mobilization nuclease domain-containing protein	-
MPG49_RS07810 (MPG49_07810)	1616738..1617052	+	315	WP_000065285.1	hypothetical protein	Antitoxin
MPG49_RS07815 (MPG49_07815)	1617045..1617326	+	282	WP_000921926.1	YafQ family addiction module toxin	Toxin
MPG49_RS07820 (MPG49_07820)	1617600..1619326	-	1727	Protein_1524	type IV secretory system conjugative DNA transfer family protein	-
MPG49_RS07825 (MPG49_07825)	1619327..1619884	-	558	WP_245052298.1	hypothetical protein	-
MPG49_RS07830 (MPG49_07830)	1619885..1620175	-	291	WP_000159825.1	hypothetical protein	-
MPG49_RS07835 (MPG49_07835)	1620172..1620627	-	456	WP_245052299.1	mobilization protein	-
MPG49_RS07840 (MPG49_07840)	1620747..1621193	-	447	WP_162966965.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1613191..1648989	35798

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10932.89 Da
• Isoelectric Point: 9.8257

>T239308 WP_000921926.1 NZ_CP094173:1617045-1617326 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSIVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T239308 NZ_LR890669:c3676688-3676371 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12121.58 Da
• Isoelectric Point: 5.2066

>AT239308 WP_000065285.1 NZ_CP094173:1616738-1617052 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT239308 NZ_LR890669:c3676378-3676091 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: K2LF18

Antitoxin

• AlphaFold DB: T5CU17