Detailed information of TA system TA023929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 256565..257259
• Replicon: chromosome
• Accession: NC_012892
• Organism: Escherichia coli BL21(DE3)

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: B21_RS01200
• Protein ID: WP_001263493.1
• Coordinates: 256861..257259 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: B21_RS01195
• Protein ID: WP_000554757.1
• Coordinates: 256565..256858 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B21_RS01175	252205..252702	+	498	WP_000006260.1	REP-associated tyrosine transposase RayT	-
B21_RS01180	252918..254630	-	1713	Protein_224	flagellar biosynthesis protein FlhA	-
B21_RS01185	254602..255387	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
B21_RS01190	255458..256513	+	1056	WP_001226164.1	DNA polymerase IV	-
B21_RS01195	256565..256858	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
B21_RS01200	256861..257259	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
B21_RS01205	257269..257721	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
B21_RS01210	257967..258170	+	204	Protein_230	RNA ligase RtcB family protein	-
B21_RS01215	258169..258690	+	522	Protein_231	peptide chain release factor H	-
B21_RS01220	258747..260204	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
B21_RS01225	260465..260923	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
B21_RS01230	261015..262259	+	1245	WP_000189541.1	esterase FrsA	-
-	261519..261599	+	81	NuclAT_13	-	-
-	261519..261599	+	81	NuclAT_13	-	-
-	261519..261599	+	81	NuclAT_13	-	-
-	261519..261599	+	81	NuclAT_13	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T23929 WP_001263493.1 NC_012892:256861-257259 [Escherichia coli BL21(DE3)]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T23929 NC_012892:256861-257259 [Escherichia coli BL21(DE3)]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT23929 WP_000554757.1 NC_012892:256565-256858 [Escherichia coli BL21(DE3)]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT23929 NC_012892:256565-256858 [Escherichia coli BL21(DE3)]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6