Detailed information of TA system TA239252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 502296..502884
• Replicon: chromosome
• Accession: NZ_CP094124
• Organism: Helicobacter pylori strain Hpfe057

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: MPG01_RS02465
• Protein ID: WP_000921941.1
• Coordinates: 502603..502884 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: T5CU17
• Locus tag: MPG01_RS02460
• Protein ID: WP_000065285.1
• Coordinates: 502296..502610 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG01_RS02440 (498335)	498335..498484	+	150	Protein_468	ParA family protein	-
MPG01_RS02445 (498566)	498566..498850	+	285	WP_180627611.1	hypothetical protein	-
MPG01_RS02450 (498882)	498882..500045	+	1164	WP_245038108.1	hypothetical protein	-
MPG01_RS02455 (500070)	500070..501999	-	1930	Protein_471	relaxase/mobilization nuclease domain-containing protein	-
MPG01_RS02460 (502296)	502296..502610	+	315	WP_000065285.1	hypothetical protein	Antitoxin
MPG01_RS02465 (502603)	502603..502884	+	282	WP_000921941.1	YafQ family addiction module toxin	Toxin
MPG01_RS02470 (503157)	503157..504883	-	1727	Protein_474	type IV secretory system conjugative DNA transfer family protein	-
MPG01_RS02475 (504884)	504884..506632	-	1749	Protein_475	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T239252 WP_000921941.1 NZ_CP094124:502603-502884 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T239252 NZ_LR890651:2162065-2162168 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12089.52 Da
• Isoelectric Point: 5.2066

>AT239252 WP_000065285.1 NZ_CP094124:502296-502610 [Helicobacter pylori]
VSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT239252 NZ_LR890651:c2162206-2161935 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: T5CU17