Detailed information of TA system TA239245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1392291..1392879
• Replicon: chromosome
• Accession: NZ_CP094119
• Organism: Helicobacter pylori strain Hpfe064

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: MPG78_RS06670
• Protein ID: WP_000921941.1
• Coordinates: 1392291..1392572 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: T5CU17
• Locus tag: MPG78_RS06675
• Protein ID: WP_000065285.1
• Coordinates: 1392565..1392879 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG78_RS06635 (MPG78_06635)	1387497..1387760	+	264	WP_220942401.1	hypothetical protein	-
MPG78_RS06640 (MPG78_06640)	1387757..1388056	+	300	WP_245040017.1	hypothetical protein	-
MPG78_RS06645 (MPG78_06645)	1388056..1388943	+	888	WP_245040020.1	ATPase, T2SS/T4P/T4SS family	-
MPG78_RS06650 (MPG78_06650)	1388993..1389448	+	456	WP_245040022.1	mobilization protein	-
MPG78_RS06655 (MPG78_06655)	1389445..1389735	+	291	WP_075658071.1	hypothetical protein	-
MPG78_RS06660 (MPG78_06660)	1389736..1390293	+	558	WP_245040024.1	hypothetical protein	-
MPG78_RS06665 (MPG78_06665)	1390294..1392021	+	1728	WP_219026171.1	type IV secretory system conjugative DNA transfer family protein	-
MPG78_RS06670 (MPG78_06670)	1392291..1392572	-	282	WP_000921941.1	YafQ family addiction module toxin	Toxin
MPG78_RS06675 (MPG78_06675)	1392565..1392879	-	315	WP_000065285.1	hypothetical protein	Antitoxin
MPG78_RS06680 (MPG78_06680)	1393172..1395083	+	1912	Protein_1300	relaxase/mobilization nuclease domain-containing protein	-
MPG78_RS06685 (MPG78_06685)	1395108..1396271	-	1164	WP_245040025.1	hypothetical protein	-
MPG78_RS06690 (MPG78_06690)	1396303..1396587	-	285	WP_000394639.1	hypothetical protein	-
MPG78_RS06695 (MPG78_06695)	1396672..1397328	-	657	WP_245040026.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1368494..1423684	55190

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T239245 WP_000921941.1 NZ_CP094119:c1392572-1392291 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T239245 NZ_LR890651:267549-267824 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12121.58 Da
• Isoelectric Point: 5.2066

>AT239245 WP_000065285.1 NZ_CP094119:c1392879-1392565 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT239245 NZ_LR890651:267821-268180 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: T5CU17