Detailed information of TA system TA239225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1146014..1146602
• Replicon: chromosome
• Accession: NZ_CP094096
• Organism: Helicobacter pylori strain Hpfe082

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: M3P300
• Locus tag: MPG69_RS05445
• Protein ID: WP_000921941.1
• Coordinates: 1146014..1146295 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: T5CU17
• Locus tag: MPG69_RS05450
• Protein ID: WP_000065285.1
• Coordinates: 1146288..1146602 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG69_RS05410 (MPG69_05410)	1141215..1141478	+	264	WP_245055306.1	hypothetical protein	-
MPG69_RS05415 (MPG69_05415)	1141475..1141774	+	300	WP_245055309.1	hypothetical protein	-
MPG69_RS05420 (MPG69_05420)	1141774..1142715	+	942	WP_245055312.1	ATPase, T2SS/T4P/T4SS family	-
MPG69_RS05425 (MPG69_05425)	1142712..1143167	+	456	WP_245055314.1	mobilization protein	-
MPG69_RS05430 (MPG69_05430)	1143164..1143454	+	291	WP_000159828.1	hypothetical protein	-
MPG69_RS05435 (MPG69_05435)	1143455..1144012	+	558	WP_245055316.1	hypothetical protein	-
MPG69_RS05440 (MPG69_05440)	1144013..1145740	+	1728	WP_245055318.1	type IV secretory system conjugative DNA transfer family protein	-
MPG69_RS05445 (MPG69_05445)	1146014..1146295	-	282	WP_000921941.1	YafQ family addiction module toxin	Toxin
MPG69_RS05450 (MPG69_05450)	1146288..1146602	-	315	WP_000065285.1	hypothetical protein	Antitoxin
MPG69_RS05455 (MPG69_05455)	1146899..1148806	+	1908	WP_245055326.1	relaxase/mobilization nuclease domain-containing protein	-
MPG69_RS05460 (MPG69_05460)	1148831..1149994	-	1164	WP_245055328.1	hypothetical protein	-
MPG69_RS05465 (MPG69_05465)	1150026..1150310	-	285	WP_000394639.1	hypothetical protein	-
MPG69_RS05470 (MPG69_05470)	1150387..1151043	-	657	WP_000587395.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10891.80 Da
• Isoelectric Point: 9.5726

>T239225 WP_000921941.1 NZ_CP094096:c1146295-1146014 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T239225 NZ_LR890643:c4534656-4534123 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATAAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12121.58 Da
• Isoelectric Point: 5.2066

>AT239225 WP_000065285.1 NZ_CP094096:c1146602-1146288 [Helicobacter pylori]
MSNIITDYSQYNEKQLHNFLNAIEKQLLKAEGDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT239225 NZ_LR890643:c4534933-4534667 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: M3P300

Antitoxin

• AlphaFold DB: T5CU17