Detailed information of TA system TA239221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 56937..57525
• Replicon: chromosome
• Accession: NZ_CP094094
• Organism: Helicobacter pylori strain Hpfe084

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: T5CW43
• Locus tag: MPG19_RS00255
• Protein ID: WP_000921929.1
• Coordinates: 56937..57218 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: MPG19_RS00260
• Protein ID: WP_180463763.1
• Coordinates: 57211..57525 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPG19_RS00220 (MPG19_00220)	52138..52401	+	264	WP_000564647.1	hypothetical protein	-
MPG19_RS00225 (MPG19_00225)	52398..52697	+	300	WP_245066209.1	hypothetical protein	-
MPG19_RS00230 (MPG19_00230)	52697..53638	+	942	WP_154547739.1	ATPase, T2SS/T4P/T4SS family	-
MPG19_RS00235 (MPG19_00235)	53635..54090	+	456	WP_245066211.1	mobilization protein	-
MPG19_RS00240 (MPG19_00240)	54087..54377	+	291	WP_245066213.1	hypothetical protein	-
MPG19_RS00245 (MPG19_00245)	54378..54935	+	558	WP_245066215.1	hypothetical protein	-
MPG19_RS00250 (MPG19_00250)	54936..56663	+	1728	WP_245066217.1	type IV secretory system conjugative DNA transfer family protein	-
MPG19_RS00255 (MPG19_00255)	56937..57218	-	282	WP_000921929.1	YafQ family addiction module toxin	Toxin
MPG19_RS00260 (MPG19_00260)	57211..57525	-	315	WP_180463763.1	hypothetical protein	Antitoxin
MPG19_RS00265 (MPG19_00265)	57820..59730	+	1911	WP_245066219.1	relaxase/mobilization nuclease domain-containing protein	-
MPG19_RS00270 (MPG19_00270)	59755..60918	-	1164	WP_245066221.1	hypothetical protein	-
MPG19_RS00275 (MPG19_00275)	60950..61234	-	285	WP_000394639.1	hypothetical protein	-
MPG19_RS00280 (MPG19_00280)	61316..61972	-	657	WP_245066223.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10904.84 Da
• Isoelectric Point: 9.8257

>T239221 WP_000921929.1 NZ_CP094094:c57218-56937 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSVVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEVKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T239221 NZ_LR890643:819297-819707 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12294.71 Da
• Isoelectric Point: 4.9533

>AT239221 WP_180463763.1 NZ_CP094094:c57525-57211 [Helicobacter pylori]
MSNIITDYSQYNEEQLHNFLNAIEKQWLKAERDKNKAIKKIQECELQEQMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT239221 NZ_LR890643:819050-819316 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: T5CW43