Detailed information of TA system TA239164

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5226609..5227204
• Replicon: chromosome
• Accession: NZ_CP093966
• Organism: Pseudomonas aeruginosa strain ATCC BAA-2114

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: MO982_RS24285
• Protein ID: WP_003117425.1
• Coordinates: 5226926..5227204 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MO982_RS24280
• Protein ID: WP_093976295.1
• Coordinates: 5226609..5226914 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO982_RS24245 (MO982_24240)	5221748..5222596	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
MO982_RS24255 (MO982_24250)	5222763..5223704	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
MO982_RS24260 (MO982_24255)	5223821..5224435	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
MO982_RS24265 (MO982_24260)	5224477..5225061	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
MO982_RS24270 (MO982_24265)	5225102..5226202	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
MO982_RS24280 (MO982_24275)	5226609..5226914	-	306	WP_093976295.1	HigA family addiction module antitoxin	Antitoxin
MO982_RS24285 (MO982_24280)	5226926..5227204	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MO982_RS24290 (MO982_24285)	5227257..5227385	-	129	Protein_4804	integrase	-
MO982_RS24295 (MO982_24290)	5227533..5229761	+	2229	WP_003113525.1	TonB-dependent receptor	-
MO982_RS24300 (MO982_24295)	5229831..5230478	-	648	WP_003095021.1	carbonate dehydratase	-
MO982_RS24305 (MO982_24300)	5230540..5231778	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T239164 WP_003117425.1 NZ_CP093966:c5227204-5226926 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T239164 NZ_LR890620:4005500-4005718 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11215.75 Da
• Isoelectric Point: 5.0747

>AT239164 WP_093976295.1 NZ_CP093966:c5226914-5226609 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWVNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT239164 NZ_LR890620:4005099-4005473 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5