Detailed information of TA system TA239159

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5351093..5351688
• Replicon: chromosome
• Accession: NZ_CP093965
• Organism: Pseudomonas aeruginosa strain ATCC BAA-2108

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: MO979_RS24735
• Protein ID: WP_003113526.1
• Coordinates: 5351410..5351688 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MO979_RS24730
• Protein ID: WP_003099268.1
• Coordinates: 5351093..5351398 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO979_RS24700 (MO979_24695)	5346546..5346836	-	291	WP_023083237.1	DUF5447 family protein	-
MO979_RS24705 (MO979_24700)	5347048..5347320	-	273	WP_003115921.1	hypothetical protein	-
MO979_RS24710 (MO979_24705)	5347430..5347696	+	267	WP_016852153.1	hypothetical protein	-
MO979_RS24715 (MO979_24710)	5347828..5348664	+	837	WP_223656480.1	helix-turn-helix domain-containing protein	-
MO979_RS24720 (MO979_24715)	5348639..5350177	+	1539	WP_079759796.1	GNAT family N-acetyltransferase	-
MO979_RS24725 (MO979_24720)	5350189..5350716	-	528	WP_071535723.1	ATP-binding protein	-
MO979_RS24730 (MO979_24725)	5351093..5351398	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
MO979_RS24735 (MO979_24730)	5351410..5351688	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MO979_RS24740 (MO979_24735)	5351741..5351869	-	129	Protein_4897	integrase	-
MO979_RS24745 (MO979_24740)	5352017..5354245	+	2229	WP_003113525.1	TonB-dependent receptor	-
MO979_RS24750 (MO979_24745)	5354315..5354962	-	648	WP_003095021.1	carbonate dehydratase	-
MO979_RS24755 (MO979_24750)	5355024..5356262	-	1239	WP_003111578.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T239159 WP_003113526.1 NZ_CP093965:c5351688-5351410 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T239159 NZ_LR890620:334263-334631 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT239159 WP_003099268.1 NZ_CP093965:c5351398-5351093 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT239159 NZ_LR890620:334045-334266 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: V6ALY3