Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 154992..155527 Replicon plasmid pNY11066-1
Accession NZ_CP093948
Organism Enterococcus faecium strain NY11066

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag MPL63_RS15120 Protein ID WP_002305108.1
Coordinates 154992..155264 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag MPL63_RS15125 Protein ID WP_002303154.1
Coordinates 155264..155527 (-) Length 88 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPL63_RS15090 (MPL63_15090) 149998..151185 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
MPL63_RS15095 (MPL63_15095) 151373..152320 + 948 WP_002303155.1 sugar-binding domain-containing protein -
MPL63_RS15100 (MPL63_15100) 152617..153165 - 549 WP_002292681.1 hypothetical protein -
MPL63_RS15105 (MPL63_15105) 153166..154023 - 858 WP_002292680.1 ParA family protein -
MPL63_RS15110 (MPL63_15110) 154309..154596 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
MPL63_RS15115 (MPL63_15115) 154586..154915 - 330 WP_002292678.1 AbrB family transcriptional regulator -
MPL63_RS15120 (MPL63_15120) 154992..155264 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
MPL63_RS15125 (MPL63_15125) 155264..155527 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MPL63_RS15130 (MPL63_15130) 155633..155956 - 324 WP_224453712.1 hypothetical protein -
MPL63_RS15135 (MPL63_15135) 156055..156618 - 564 Protein_160 IS6-like element ISS1N family transposase -
MPL63_RS15140 (MPL63_15140) 156698..157860 + 1163 WP_112020409.1 IS3 family transposase -
MPL63_RS15145 (MPL63_15145) 158332..159231 - 900 WP_002303235.1 aldose 1-epimerase family protein -
MPL63_RS15150 (MPL63_15150) 159252..159524 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..210381 210381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-87)

Antitoxin

(5-69)


Sequences


Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T239130 WP_002305108.1 NZ_CP093948:c155264-154992 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T239130 NZ_LR890606:c4159162-4158788 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT239130 WP_002303154.1 NZ_CP093948:c155527-155264 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT239130 NZ_LR890606:c4159620-4159252 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCTCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A133CER3


Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References