Detailed information of TA system TA239058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 62967..63678
• Replicon: plasmid pGA_Eco1
• Accession: NZ_CP093921
• Organism: Escherichia coli strain ST167

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: MOO49_RS24335
• Protein ID: WP_000162415.1
• Coordinates: 63376..63678 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MOO49_RS24330
• Protein ID: WP_000806445.1
• Coordinates: 62967..63305 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO49_RS24300	58262..59083	-	822	WP_001427910.1	hypothetical protein	-
MOO49_RS24305	59429..60082	+	654	WP_000410952.1	hypothetical protein	-
MOO49_RS24310	60411..60740	+	330	WP_000542383.1	hypothetical protein	-
MOO49_RS24315	60733..61926	+	1194	WP_102218742.1	phage tail protein	-
MOO49_RS24320	61960..62688	-	729	WP_000986754.1	hypothetical protein	-
MOO49_RS24325	62725..62910	-	186	Protein_81	hypothetical protein	-
MOO49_RS24330	62967..63305	-	339	WP_000806445.1	HigA family addiction module antitoxin	Antitoxin
MOO49_RS24335	63376..63678	-	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOO49_RS24340	63841..64632	+	792	WP_000532305.1	hypothetical protein	-
MOO49_RS24345	64629..65396	+	768	WP_000203290.1	hypothetical protein	-
MOO49_RS24350	65400..66380	+	981	WP_000046499.1	hypothetical protein	-
MOO49_RS24355	66377..67030	+	654	WP_000828873.1	hypothetical protein	-
MOO49_RS24360	67090..67995	+	906	WP_001258014.1	recombination-associated protein RdgC	-
MOO49_RS24365	68009..68659	+	651	WP_032153356.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90296	90296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T239058 WP_000162415.1 NZ_CP093921:c63678-63376 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T239058 NZ_LR890584:13931-14347 [Klebsiella pneumoniae]
GTGAACAAAATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATTACCTACGCCGAGATGCGCTTCGGCGCCACCGGGC
CGAAGGCCGCGCCGCGTCACGTCCAGCTGGTTGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGCATGGCCGGCACGCCGATTGGCCCGAACGACACGGCGATTGC
CGGACACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT239058 WP_000806445.1 NZ_CP093921:c63305-62967 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT239058 NZ_LR890584:13704-13934 [Klebsiella pneumoniae]
ATGCGCACAGTCTCTATTTTTAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGTTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3