Detailed information of TA system TA239053

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86230..86484
• Replicon: plasmid pGA_EcoNDM5
• Accession: NZ_CP093920
• Organism: Escherichia coli strain ST167

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MOO49_RS23800
• Protein ID: WP_001312851.1
• Coordinates: 86335..86484 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	86230..86291 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOO49_RS23765 (81882)	81882..82094	+	213	WP_005012601.1	hypothetical protein	-
MOO49_RS23770 (82395)	82395..82484	-	90	Protein_93	IS1 family transposase	-
MOO49_RS23775 (82539)	82539..83216	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
MOO49_RS23780 (83216)	83216..83563	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
MOO49_RS23785 (83583)	83583..85154	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
MOO49_RS23790 (85192)	85192..85808	-	617	Protein_97	IS1-like element IS1A family transposase	-
MOO49_RS23795 (85909)	85909..86091	+	183	WP_000968309.1	hypothetical protein	-
- (86230)	86230..86291	-	62	NuclAT_0	-	Antitoxin
- (86230)	86230..86291	-	62	NuclAT_0	-	Antitoxin
- (86230)	86230..86291	-	62	NuclAT_0	-	Antitoxin
- (86230)	86230..86291	-	62	NuclAT_0	-	Antitoxin
MOO49_RS23800 (86335)	86335..86484	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
MOO49_RS23805 (86768)	86768..87025	+	258	WP_000083833.1	replication regulatory protein RepA	-
MOO49_RS23810 (87261)	87261..87335	+	75	WP_032336874.1	RepA leader peptide Tap	-
MOO49_RS23815 (87328)	87328..87774	+	447	Protein_102	plasmid replication initiator RepA	-
MOO49_RS23820 (87774)	87774..88388	-	615	Protein_103	VENN motif pre-toxin domain-containing protein	-
MOO49_RS23825 (89095)	89095..90315	+	1221	WP_000410951.1	arginine deiminase	-
MOO49_RS23830 (90326)	90326..91237	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / aac(3)-IIa / mph(A) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..100291	100291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T239053 WP_001312851.1 NZ_CP093920:86335-86484 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T239053 NZ_LR890583:4005438-4005656 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT239053 NZ_CP093920:c86291-86230 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin