Detailed information of TA system TA239046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3786801..3787495
• Replicon: chromosome
• Accession: NZ_CP093919
• Organism: Escherichia coli strain ST167

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: MOO49_RS18250
• Protein ID: WP_001263489.1
• Coordinates: 3786801..3787199 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MOO49_RS18255
• Protein ID: WP_000554758.1
• Coordinates: 3787202..3787495 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3782389)	3782389..3782469	-	81	NuclAT_12	-	-
- (3782389)	3782389..3782469	-	81	NuclAT_12	-	-
- (3782389)	3782389..3782469	-	81	NuclAT_12	-	-
- (3782389)	3782389..3782469	-	81	NuclAT_12	-	-
MOO49_RS18225 (3783065)	3783065..3783523	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
MOO49_RS18230 (3783784)	3783784..3785241	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
MOO49_RS18235 (3785298)	3785298..3785819	-	522	Protein_3591	peptide chain release factor H	-
MOO49_RS18240 (3785815)	3785815..3786021	-	207	Protein_3592	RtcB family protein	-
MOO49_RS18245 (3786339)	3786339..3786791	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
MOO49_RS18250 (3786801)	3786801..3787199	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MOO49_RS18255 (3787202)	3787202..3787495	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MOO49_RS18260 (3787547)	3787547..3788602	-	1056	WP_001226164.1	DNA polymerase IV	-
MOO49_RS18265 (3788673)	3788673..3789458	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
MOO49_RS18270 (3789430)	3789430..3791142	+	1713	Protein_3598	flagellar biosynthesis protein FlhA	-
MOO49_RS18275 (3791366)	3791366..3791863	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T239046 WP_001263489.1 NZ_CP093919:c3787199-3786801 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T239046 NZ_LR890583:331912-332280 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT239046 WP_000554758.1 NZ_CP093919:c3787495-3787202 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT239046 NZ_LR890583:331694-331915 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3