Detailed information of TA system TA023903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 839695..840309
• Replicon: chromosome
• Accession: NC_012850
• Organism: Rhizobium leguminosarum bv. trifolii WSM1325

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C6ARV7
• Locus tag: RLEG_RS04130
• Protein ID: WP_012756493.1
• Coordinates: 839695..839991 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: RLEG_RS04135
• Protein ID: WP_012756494.1
• Coordinates: 840001..840309 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RLEG_RS04105	835237..835599	+	363	WP_012756488.1	helix-turn-helix domain-containing protein	-
RLEG_RS04110	835580..836074	+	495	WP_012756489.1	SRPBCC family protein	-
RLEG_RS04115	836140..836874	-	735	WP_012756490.1	phosphotransferase	-
RLEG_RS04120	836992..838923	-	1932	WP_012756491.1	adenylate/guanylate cyclase domain-containing protein	-
RLEG_RS04125	839280..839558	-	279	WP_026157280.1	hypothetical protein	-
RLEG_RS04130	839695..839991	+	297	WP_012756493.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RLEG_RS04135	840001..840309	+	309	WP_012756494.1	HigA family addiction module antidote protein	Antitoxin
RLEG_RS04140	840438..840785	+	348	WP_012756495.1	hypothetical protein	-
RLEG_RS04145	840787..841140	+	354	WP_012756496.1	hypothetical protein	-
RLEG_RS04150	841176..841754	-	579	WP_164902758.1	DUF308 domain-containing protein	-
RLEG_RS04155	841991..842620	-	630	WP_012756498.1	hypothetical protein	-
RLEG_RS04160	842692..843282	-	591	WP_012756499.1	TetR/AcrR family transcriptional regulator	-
RLEG_RS04165	843481..844197	+	717	WP_012756500.1	SOS response-associated peptidase	-
RLEG_RS04170	844272..844592	+	321	WP_012756501.1	hypothetical protein	-
RLEG_RS04175	844589..844828	+	240	WP_012756502.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.14 Da
• Isoelectric Point: 6.0777

>T23903 WP_012756493.1 NC_012850:839695-839991 [Rhizobium leguminosarum bv. trifolii WSM1325]
MIVGFQDEWLRAFFVEDIHSRNIPSDLESRLFRKLQMIDDAMTDQDLRVPPSNHFEKLRGTLDGLHSIRVNKQWRLIFRW
DGGRGEASDVYLDDHSYR

Nucleotide

• Length: 297 bp

>T23903 NC_012850:839695-839991 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGATCGTCGGGTTTCAGGACGAATGGCTGCGGGCCTTTTTCGTGGAGGATATCCACTCCCGCAACATTCCGTCCGATCT
CGAAAGCCGGTTGTTCCGCAAGCTCCAGATGATCGACGACGCGATGACCGATCAGGATTTGCGTGTGCCACCCAGCAATC
ATTTCGAGAAGCTGCGCGGCACGCTCGACGGCCTGCATTCGATCCGCGTCAACAAGCAGTGGCGGCTGATCTTCCGATGG
GACGGCGGTCGCGGCGAAGCGTCGGATGTTTATCTCGACGACCACAGCTATCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11423.20 Da
• Isoelectric Point: 10.2965

>AT23903 WP_012756494.1 NC_012850:840001-840309 [Rhizobium leguminosarum bv. trifolii WSM1325]
MLMTTRKPATVGEILTEEFMQPLGLTQAVLAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNTQRRSDLW
SVMNSPEERARVDRAKPLAKAA

Nucleotide

• Length: 309 bp

>AT23903 NC_012850:840001-840309 [Rhizobium leguminosarum bv. trifolii WSM1325]
ATGTTGATGACCACACGCAAACCGGCCACGGTCGGCGAAATCCTGACGGAAGAGTTCATGCAGCCGCTCGGCCTGACGCA
GGCGGTGCTTGCCGAGGCGATGGGCGTGCAGCGCAAGCATGTGAACGAACTCTGCAACGACCGGCGCAACGTGACGGCGG
CGACCGCGCTCATTCTGGCGCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTGAACACGCAGCGTCGCAGCGATCTGTGG
AGCGTCATGAATTCGCCCGAGGAGCGGGCGCGGGTCGATCGTGCCAAGCCCTTGGCGAAGGCGGCATAG

Structures

Toxin

• AlphaFold DB: C6ARV7