Detailed information of TA system TA239021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3559170..3559827
• Replicon: chromosome
• Accession: NZ_CP093914
• Organism: Enterobacter cloacae strain SD21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A421IAD7
• Locus tag: MQH03_RS16660
• Protein ID: WP_014832979.1
• Coordinates: 3559170..3559469 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MQH03_RS16665
• Protein ID: WP_038980942.1
• Coordinates: 3559480..3559827 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MQH03_RS16645 (MQH03_16645)	3554783..3556975	+	2193	WP_061771961.1	type I secretion system permease/ATPase	-
MQH03_RS16650 (MQH03_16650)	3556986..3558155	+	1170	WP_086528237.1	HlyD family efflux transporter periplasmic adaptor subunit	-
MQH03_RS16655 (MQH03_16655)	3558479..3558916	+	438	WP_080453047.1	hypothetical protein	-
MQH03_RS16660 (MQH03_16660)	3559170..3559469	+	300	WP_014832979.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MQH03_RS16665 (MQH03_16665)	3559480..3559827	+	348	WP_038980942.1	HigA family addiction module antitoxin	Antitoxin
MQH03_RS16670 (MQH03_16670)	3559877..3560002	+	126	Protein_3259	nucleoside triphosphatase NudI	-
MQH03_RS16675 (MQH03_16675)	3559974..3560705	-	732	WP_038980944.1	helix-turn-helix transcriptional regulator	-
MQH03_RS16680 (MQH03_16680)	3560804..3561223	+	420	WP_061771959.1	nuclear transport factor 2 family protein	-
MQH03_RS16685 (MQH03_16685)	3561226..3561744	-	519	WP_061773058.1	hypothetical protein	-
MQH03_RS16690 (MQH03_16690)	3561830..3563011	-	1182	WP_061771958.1	PLP-dependent aminotransferase family protein	-
MQH03_RS16695 (MQH03_16695)	3563031..3563918	-	888	WP_040023513.1	LysR family transcriptional regulator	-
MQH03_RS16700 (MQH03_16700)	3564016..3564618	+	603	WP_061771957.1	short chain dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11646.28 Da
• Isoelectric Point: 10.0182

>T239021 WP_014832979.1 NZ_CP093914:3559170-3559469 [Enterobacter cloacae]
MINHFRDQWLEDFFLYGRSSNVIPSNLETVLARKLDLINAAISHRDLRSPPGNMFEALNPPLKGYSSIRVNRQYRLVFRW
AEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T239021 NZ_LR890564:c5167903-5167520 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGCCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13018.00 Da
• Isoelectric Point: 7.0139

>AT239021 WP_038980942.1 NZ_CP093914:3559480-3559827 [Enterobacter cloacae]
MILQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVAEVIAKRKGGQPDVA

Nucleotide

• Length: 348 bp

>AT239021 NZ_LR890564:c5168145-5167903 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A421IAD7