238924

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 356072..356669 Replicon chromosome
Accession NZ_CP093854
Organism Klebsiella pneumoniae strain Bio45

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MOQ73_RS01710 Protein ID WP_004142563.1
Coordinates 356072..356389 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MOQ73_RS01715 Protein ID WP_004142561.1
Coordinates 356382..356669 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ73_RS01695 (351775) 351775..353202 - 1428 WP_004176980.1 MFS transporter -
MOQ73_RS01700 (353311) 353311..354177 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
MOQ73_RS01705 (354844) 354844..355887 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
MOQ73_RS01710 (356072) 356072..356389 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MOQ73_RS01715 (356382) 356382..356669 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MOQ73_RS01720 (356937) 356937..357587 - 651 Protein_339 oxygen-insensitive NAD(P)H nitroreductase -
MOQ73_RS01725 (357707) 357707..358075 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
MOQ73_RS01730 (358075) 358075..358656 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
MOQ73_RS01735 (358836) 358836..359951 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
MOQ73_RS01740 (359982) 359982..360323 + 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
MOQ73_RS01745 (360341) 360341..360589 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T238924 WP_004142563.1 NZ_CP093854:356072-356389 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T238924 NZ_LR890536:3386786-3387172 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGTAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT238924 WP_004142561.1 NZ_CP093854:356382-356669 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT238924 NZ_LR890536:3387162-3387491 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References