Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 825626..826223 Replicon chromosome
Accession NZ_CP093852
Organism Klebsiella pneumoniae strain Bio73

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MOQ71_RS03905 Protein ID WP_004142563.1
Coordinates 825626..825943 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MOQ71_RS03910 Protein ID WP_004142561.1
Coordinates 825936..826223 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MOQ71_RS03890 (821329) 821329..822756 - 1428 WP_004176980.1 MFS transporter -
MOQ71_RS03895 (822865) 822865..823731 + 867 WP_004178894.1 helix-turn-helix transcriptional regulator -
MOQ71_RS03900 (824398) 824398..825441 + 1044 WP_004178895.1 DUF2157 domain-containing protein -
MOQ71_RS03905 (825626) 825626..825943 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MOQ71_RS03910 (825936) 825936..826223 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MOQ71_RS03915 (826491) 826491..827141 - 651 Protein_767 oxygen-insensitive NAD(P)H nitroreductase -
MOQ71_RS03920 (827261) 827261..827629 - 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
MOQ71_RS03925 (827629) 827629..828210 - 582 WP_043906838.1 TetR/AcrR family transcriptional regulator -
MOQ71_RS03930 (828390) 828390..829505 + 1116 WP_020316605.1 MBL fold metallo-hydrolase -
MOQ71_RS03935 (829536) 829536..829877 + 342 WP_166689814.1 RamA family antibiotic efflux transcriptional regulator -
MOQ71_RS03940 (829895) 829895..830143 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T238899 WP_004142563.1 NZ_CP093852:825626-825943 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T238899 NZ_LR890527:c4450359-4449826 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATATGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCTGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT238899 WP_004142561.1 NZ_CP093852:825936-826223 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT238899 NZ_LR890527:c4450636-4450370 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References