Detailed information of TA system TA238899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 825626..826223
• Replicon: chromosome
• Accession: NZ_CP093852
• Organism: Klebsiella pneumoniae strain Bio73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4YIC5
• Locus tag: MOQ71_RS03905
• Protein ID: WP_004142563.1
• Coordinates: 825626..825943 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: MOQ71_RS03910
• Protein ID: WP_004142561.1
• Coordinates: 825936..826223 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MOQ71_RS03890 (821329)	821329..822756	-	1428	WP_004176980.1	MFS transporter	-
MOQ71_RS03895 (822865)	822865..823731	+	867	WP_004178894.1	helix-turn-helix transcriptional regulator	-
MOQ71_RS03900 (824398)	824398..825441	+	1044	WP_004178895.1	DUF2157 domain-containing protein	-
MOQ71_RS03905 (825626)	825626..825943	+	318	WP_004142563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MOQ71_RS03910 (825936)	825936..826223	+	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
MOQ71_RS03915 (826491)	826491..827141	-	651	Protein_767	oxygen-insensitive NAD(P)H nitroreductase	-
MOQ71_RS03920 (827261)	827261..827629	-	369	WP_004142557.1	MmcQ/YjbR family DNA-binding protein	-
MOQ71_RS03925 (827629)	827629..828210	-	582	WP_043906838.1	TetR/AcrR family transcriptional regulator	-
MOQ71_RS03930 (828390)	828390..829505	+	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
MOQ71_RS03935 (829536)	829536..829877	+	342	WP_166689814.1	RamA family antibiotic efflux transcriptional regulator	-
MOQ71_RS03940 (829895)	829895..830143	-	249	WP_002893037.1	DUF1158 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T238899 WP_004142563.1 NZ_CP093852:825626-825943 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T238899 NZ_LR890527:c4450359-4449826 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATATGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCTGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT238899 WP_004142561.1 NZ_CP093852:825936-826223 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT238899 NZ_LR890527:c4450636-4450370 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0M5MXH8

Antitoxin

• AlphaFold DB: A0A0S3DIQ1