Detailed information of TA system TA238893

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 31700..32121
• Replicon: plasmid unnamed2
• Accession: NZ_CP093550
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MPJ80_RS25235
• Protein ID: WP_223412041.1
• Coordinates: 31999..32121 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	31700..31907 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS25195 (26928)	26928..27101	-	174	Protein_33	hypothetical protein	-
MPJ80_RS25200 (27403)	27403..27924	+	522	WP_012881138.1	single-stranded DNA-binding protein	-
MPJ80_RS25205 (27980)	27980..28213	+	234	WP_000005971.1	DUF905 domain-containing protein	-
MPJ80_RS25210 (28277)	28277..30235	+	1959	WP_012881137.1	ParB/RepB/Spo0J family partition protein	-
MPJ80_RS25215 (30290)	30290..30724	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
MPJ80_RS25220 (30721)	30721..31440	+	720	WP_012881136.1	plasmid SOS inhibition protein A	-
MPJ80_RS25225 (31437)	31437..31751	+	315	WP_000872086.1	hypothetical protein	-
- (31700)	31700..31907	+	208	NuclAT_0	-	Antitoxin
- (31700)	31700..31907	+	208	NuclAT_0	-	Antitoxin
- (31700)	31700..31907	+	208	NuclAT_0	-	Antitoxin
- (31700)	31700..31907	+	208	NuclAT_0	-	Antitoxin
MPJ80_RS25230 (31941)	31941..32054	+	114	Protein_40	DUF5431 family protein	-
MPJ80_RS25235 (31999)	31999..32121	+	123	WP_223412041.1	Hok/Gef family protein	Toxin
MPJ80_RS25240 (32422)	32422..32671	-	250	Protein_42	hypothetical protein	-
MPJ80_RS25245 (32967)	32967..33290	+	324	WP_000533253.1	hypothetical protein	-
MPJ80_RS25250 (33349)	33349..33591	+	243	WP_000540588.1	hypothetical protein	-
MPJ80_RS25255 (33791)	33791..33943	-	153	Protein_45	hypothetical protein	-
MPJ80_RS25260 (34056)	34056..35723	-	1668	WP_117313998.1	group II intron reverse transcriptase/maturase	-
MPJ80_RS25265 (36452)	36452..36625	-	174	Protein_47	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..79537	79537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4560.33 Da
• Isoelectric Point: 8.2774

>T238893 WP_223412041.1 NZ_CP093550:31999-32121 [Escherichia coli]
IVCCTLLIFTHLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T238893 NZ_LR890527:727232-727717 [Klebsiella pneumoniae]
ATGATCTTGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTAGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 208 bp

>AT238893 NZ_CP093550:31700-31907 [Escherichia coli]
ACTGATTGCCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGCAGAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin