Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4387885..4388579 Replicon chromosome
Accession NZ_CP093548
Organism Escherichia coli strain YL03

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag MPJ80_RS21230 Protein ID WP_001521903.1
Coordinates 4388181..4388579 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag MPJ80_RS21225 Protein ID WP_000554758.1
Coordinates 4387885..4388178 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPJ80_RS21200 (4383000) 4383000..4383749 + 750 WP_016233951.1 C40 family peptidase -
MPJ80_RS21205 (4383806) 4383806..4384159 - 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
MPJ80_RS21210 (4384238) 4384238..4385950 - 1713 Protein_4142 flagellar biosynthesis protein FlhA -
MPJ80_RS21215 (4385922) 4385922..4386707 + 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
MPJ80_RS21220 (4386778) 4386778..4387833 + 1056 WP_001226177.1 DNA polymerase IV -
MPJ80_RS21225 (4387885) 4387885..4388178 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
MPJ80_RS21230 (4388181) 4388181..4388579 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
MPJ80_RS21235 (4388589) 4388589..4389041 + 453 WP_023144376.1 GNAT family N-acetyltransferase -
MPJ80_RS21240 (4389286) 4389286..4389492 + 207 Protein_4148 RtcB family protein -
MPJ80_RS21245 (4389488) 4389488..4389840 + 353 Protein_4149 peptide chain release factor H -
MPJ80_RS21250 (4389897) 4389897..4391354 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
MPJ80_RS21255 (4391615) 4391615..4392073 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (4392669) 4392669..4392749 + 81 NuclAT_11 - -
- (4392669) 4392669..4392749 + 81 NuclAT_11 - -
- (4392669) 4392669..4392749 + 81 NuclAT_11 - -
- (4392669) 4392669..4392749 + 81 NuclAT_11 - -
MPJ80_RS21260 (4392165) 4392165..4393409 + 1245 WP_000189541.1 esterase FrsA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T238883 WP_001521903.1 NZ_CP093548:4388181-4388579 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T238883 NZ_LR890524:587837-588733 [Burkholderia cepacia]
ATGAGTGTCATCGACAAACGTGAACGCGACCGCACCGCGGCGTTCGCCGCGCTGCTCGGGCAGTTTCCCGAGCAGCGTTG
CGCGGCCGGCGCGGCGGGCACGATCGGTTATCGCGAGGCCGGCGCGCAGCATGCGGGCCGCGCGCTGCCCGTCGTGCTGC
TGCACGGGATCGGCTCGGGCGCCGCGTCGTGGGTCCGCCAGCTCGACACGCTGGGCGCATCGCGGCGCGTGCTCGCGTGG
GATGCGCCCGGTTACGGCGTGTCGACGCCCGTGCACGGCGCGTCGCCGGCCGCCGCCGATTACGCGGCAGCGCTGAACGC
GTGGCTCGAGGCACTCGGCATCGAACGCTGCGTCCTGGTCGGCCATTCGCTCGGCGCGATCATCGCGGGCGGTCTCGCCC
GCGTGACGCCCGCGCGGATCGCCGGCCTGCTGCTGGTATCGCCCGCGGGCGGCTACGGCAGCGCACCGGCCGAGACCCGC
GAAGCGCGCCGCGACGCGCGGCTCGCGATGCTCGCGGAACTCGGCCCGGCCGGGCTCGCCGAGCAGCGCAGCGGCAGCAT
GCTGTCCGGTTTCGCGAACGAGGAGGCACGGGCGTGGGTGCGCTGGAACATGGCGCGCATCGTGCCGGCCGGCTATGCGC
AGGCCACGCATCTGCTCGCGAACGCCGATCTCGCGGCCGATCTCGCCGGCTTCCGGGGCCGCACGGCCGTGGCCGTCGGC
GCGAACGACGCGATCACGCCGCCCGCCGCGTGCGAGCGGATCGCCGCGGCCGCGCACGTCGGGCTGCAGGTGATTCCGCA
GGCAGGGCACGCCGGCTATGTGGAAGCGCCGGCCGCGTATTCCGCGCTGATCGACTCGTTCTGCCGGCAGTGCGATACGC
AGTGGGGGCTGGCATGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT238883 WP_000554758.1 NZ_CP093548:4387885-4388178 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT238883 NZ_LR890524:588772-589629 [Burkholderia cepacia]
ATGAGCGAACCGCTGCAACAAAACGAATCCGAAAACGCGAAGGCCGAAGCCAGCTACGTGGTGCCGGGCCTCGAACGCGG
GTTGCGGATTCTCGCCGAATTCTCGCCGCGCGAGCCCGTGCTCGGCGCGCCCGAATTGTCGAAGCGGCTCGGCATTCCGC
GCACGACGGTGTTCCGTCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAACGCGCGGACAAGGATCGCAACTACAAG
CTCGGCATCGCGGTGCTGCGGCTCGGCTTCGAATACCTGAGCTCGCTGGAGCTGACCGATCTCGGCCTGCCGGTGATCGA
AAGCCTGCGCGATGCGACAGGCTTCACGACGCACATCGTGATCCGCGACGGCCGCGACGTGGTGTTCGTCGCGAAGGCGC
AGAGCCAGTCGCCGGTGTTCAGTTCGATCCGCGTGAACGTCGGCACCCGGCTGCCCGCGCATGCAACGACACACGGCCAC
GTGCTGATGGGCGACCTGTCGCTGAAGGAACTGCATGCGCTGTATCCGGAAGGCGTGCTGAACCGGATGACGAGCGCGAC
GCCGGAAACGGTCGACGCGCTGTACGAAGTGATCCGCGAGGATGCGCTGCGCGGCTACGGCGTCAGCAATTCGTCGTTCG
AGCGCGGGATCTCGGTCGTCACGGCGCCGGTGCGCAACGAGACGCAGAAGATCGTCGCTTGCATCACGGTGACGGTGCCG
CGTCCGGAGATCGACGCGGCGCTGATCGCGGACGGGCTGATCGACAAGGTGCAGCGCGCGGCGGCGGAACTGTCGCGGCG
GCTGAATTACCGCTCGGATGACGAGCACACGTTTCTCAAAGCTTTAGGACTCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References