Detailed information of TA system TA238871

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2072750..2073477
• Replicon: chromosome
• Accession: NZ_CP093548
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: MPJ80_RS10220
• Protein ID: WP_001521139.1
• Coordinates: 2073166..2073477 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MPJ80_RS10215
• Protein ID: WP_000126294.1
• Coordinates: 2072750..2073169 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS10195 (2067835)	2067835..2068362	-	528	WP_001078777.1	electron transport protein HydN	-
MPJ80_RS10200 (2068511)	2068511..2069521	-	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
MPJ80_RS10205 (2069781)	2069781..2071238	+	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
MPJ80_RS10210 (2071247)	2071247..2072671	+	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
MPJ80_RS10215 (2072750)	2072750..2073169	-	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
MPJ80_RS10220 (2073166)	2073166..2073477	-	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
MPJ80_RS10225 (2073644)	2073644..2074114	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
MPJ80_RS10230 (2074107)	2074107..2074517	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
MPJ80_RS10235 (2074514)	2074514..2075281	-	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
MPJ80_RS10240 (2075281)	2075281..2075823	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
MPJ80_RS10245 (2075833)	2075833..2077542	-	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T238871 WP_001521139.1 NZ_CP093548:c2073477-2073166 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T238871 NZ_LR890515:3701342-3701725 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT238871 WP_000126294.1 NZ_CP093548:c2073169-2072750 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT238871 NZ_LR890515:3701100-3701342 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A829L2T9