Detailed information of TA system TA238870

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1336374..1336876
• Replicon: chromosome
• Accession: NZ_CP093548
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: MPJ80_RS06870
• Protein ID: WP_000767829.1
• Coordinates: 1336374..1336628 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: MPJ80_RS06875
• Protein ID: WP_001259255.1
• Coordinates: 1336625..1336876 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS06840 (1331396)	1331396..1331616	-	221	Protein_1349	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
MPJ80_RS06845 (1331630)	1331630..1332688	-	1059	WP_023568741.1	thiosulfate utilization transporter TsuA/YeeE	-
MPJ80_RS06850 (1332867)	1332867..1334225	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
MPJ80_RS06855 (1334215)	1334215..1334277	-	63	WP_010723108.1	membrane protein YoeI	-
MPJ80_RS06860 (1334492)	1334492..1335421	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
MPJ80_RS06865 (1335467)	1335467..1336291	-	825	WP_000754737.1	SDR family oxidoreductase	-
MPJ80_RS06870 (1336374)	1336374..1336628	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
MPJ80_RS06875 (1336625)	1336625..1336876	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
MPJ80_RS06880 (1337159)	1337159..1337209	+	51	WP_071593552.1	his operon leader peptide	-
MPJ80_RS06885 (1337355)	1337355..1338254	+	900	WP_000131789.1	ATP phosphoribosyltransferase	-
MPJ80_RS06890 (1338260)	1338260..1339564	+	1305	WP_039267630.1	histidinol dehydrogenase	-
MPJ80_RS06895 (1339561)	1339561..1340631	+	1071	WP_000108943.1	histidinol-phosphate transaminase	-
MPJ80_RS06900 (1340631)	1340631..1341698	+	1068	WP_000080093.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T238870 WP_000767829.1 NZ_CP093548:c1336628-1336374 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T238870 NZ_LR890515:c3287860-3287492 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT238870 WP_001259255.1 NZ_CP093548:c1336876-1336625 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT238870 NZ_LR890515:c3288078-3287857 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0