Detailed information of TA system TA238855

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 538314..538685
• Replicon: chromosome
• Accession: NZ_CP093548
• Organism: Escherichia coli strain YL03

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: MPJ80_RS02770
• Protein ID: WP_001443846.1
• Coordinates: 538536..538685 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	538314..538492 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPJ80_RS02740 (534066)	534066..534239	+	174	WP_001296046.1	protein YnaL	-
MPJ80_RS02745 (534269)	534269..535642	+	1374	WP_000123748.1	ATP-dependent RNA helicase DbpA	-
MPJ80_RS02750 (535771)	535771..536706	-	936	WP_001523172.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
MPJ80_RS02755 (536758)	536758..537993	-	1236	WP_000040839.1	site-specific integrase	-
MPJ80_RS02760 (537995)	537995..538210	-	216	WP_000079604.1	excisionase XisR	-
- (538314)	538314..538492	+	179	NuclAT_0	-	Antitoxin
- (538314)	538314..538492	+	179	NuclAT_0	-	Antitoxin
- (538314)	538314..538492	+	179	NuclAT_0	-	Antitoxin
- (538314)	538314..538492	+	179	NuclAT_0	-	Antitoxin
MPJ80_RS02765 (538310)	538310..538498	-	189	WP_001302840.1	DUF1187 family protein	-
MPJ80_RS02770 (538536)	538536..538685	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
MPJ80_RS02775 (538741)	538741..539550	-	810	WP_000166313.1	recombination protein RecT	-
MPJ80_RS02780 (539543)	539543..542143	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
MPJ80_RS02785 (542245)	542245..542520	-	276	WP_001344816.1	hypothetical protein	-
MPJ80_RS02790 (542595)	542595..542765	-	171	WP_001352098.1	YdaE family protein	-
MPJ80_RS02795 (542765)	542765..542986	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	536758..589837	53079

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T238855 WP_001443846.1 NZ_CP093548:c538685-538536 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T238855 NZ_LR890508:c2673481-2673062 [Escherichia coli]
ATGACGGATTATCAGCCATACAGGAAAGGAACTGTGCTTGCCCCAACTGGACCATGCAATCATCTTCATGTGATTTGTAA
TGATCCTGTTTATTACCCCGTTAACGATTGTTATTGTGTTTTAGTTGTTAATATTTCTAGTATCAAGGATGGTGTCCCCC
ACGATCCGTCTTGCGTCTTGAATTCTGGTGATCATCGCTTTATCAAGCATCCAAGTTATGTTGTTTACGCTGAAGCTATA
ATTTGGCGAGTGGATAACATGGTTAGAAAGCAGCGATCGGGTGAGATTTCTGTTCATGATGATATGCCAGAAGCTACATT
CAATAGAATTCTGGACGGTTTTGATATCTCTGATGAAGTTACGCCAAAGAACCTTAAATTTAAAAATAAATATTGCGTAT
CATCTATTGATGATGAGTAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT238855 NZ_CP093548:538314-538492 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin