Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 352758..353404 Replicon chromosome
Accession NZ_CP093495
Organism Klebsiella pneumoniae strain VA32

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MO414_RS01630 Protein ID WP_002920560.1
Coordinates 352758..353105 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MO414_RS01635 Protein ID WP_002920557.1
Coordinates 353105..353404 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO414_RS01620 (MO414_01620) 348684..350117 + 1434 WP_002920564.1 glycogen synthase GlgA -
MO414_RS01625 (MO414_01625) 350135..352582 + 2448 WP_002920561.1 glycogen phosphorylase -
MO414_RS01630 (MO414_01630) 352758..353105 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MO414_RS01635 (MO414_01635) 353105..353404 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MO414_RS01640 (MO414_01640) 353467..354975 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MO414_RS01645 (MO414_01645) 355180..355509 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MO414_RS01650 (MO414_01650) 355560..356390 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MO414_RS01655 (MO414_01655) 356440..357197 + 758 Protein_327 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T238775 WP_002920560.1 NZ_CP093495:352758-353105 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T238775 NZ_LR890471:c4745741-4745400 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGACGCTATATCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACTCTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTATGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT238775 WP_002920557.1 NZ_CP093495:353105-353404 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT238775 NZ_LR890471:c4746103-4745762 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTTTAAAATGGGGACTGAATTGTAATATGACACCTTGCTTTGGTGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGATCGGGCCTGCATTACCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCACACGTTTTATCCGCCAAATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCAT
GCCCACAGTGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
CGTTTACCCCACTCATCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References