Detailed information of TA system TA238708

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51784..52038
• Replicon: plasmid pVA569-78
• Accession: NZ_CP093477
• Organism: Klebsiella pneumoniae strain VA569

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: MO417_RS28080
• Protein ID: WP_001312851.1
• Coordinates: 51784..51933 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	51977..52038 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO417_RS28050 (47031)	47031..47942	-	912	WP_000440183.1	carbamate kinase	-
MO417_RS28055 (47953)	47953..49173	-	1221	WP_000410951.1	arginine deiminase	-
MO417_RS28060 (49880)	49880..50494	+	615	Protein_61	VENN motif pre-toxin domain-containing protein	-
MO417_RS28065 (50494)	50494..50940	-	447	Protein_62	plasmid replication initiator RepA	-
MO417_RS28070 (50933)	50933..51007	-	75	WP_032336874.1	RepA leader peptide Tap	-
MO417_RS28075 (51243)	51243..51500	-	258	WP_000083833.1	replication regulatory protein RepA	-
MO417_RS28080 (51784)	51784..51933	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (51977)	51977..52038	+	62	NuclAT_0	-	Antitoxin
- (51977)	51977..52038	+	62	NuclAT_0	-	Antitoxin
- (51977)	51977..52038	+	62	NuclAT_0	-	Antitoxin
- (51977)	51977..52038	+	62	NuclAT_0	-	Antitoxin
MO417_RS28090 (53021)	53021..53491	-	471	WP_283462741.1	reverse transcriptase/maturase family protein	-
MO417_RS28095 (53552)	53552..54334	-	783	WP_001317493.1	IS21-like element IS100kyp family helper ATPase IstB	-
MO417_RS28100 (54331)	54331..55353	-	1023	WP_000255956.1	IS21-like element IS100 family transposase	-
MO417_RS28105 (55380)	55380..55835	-	456	WP_283462684.1	hypothetical protein	-
MO417_RS28110 (56513)	56513..56719	-	207	WP_000275856.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / sul1 / qacE / aadA5 / aac(3)-IId / blaTEM-1B	-	1..77813	77813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T238708 WP_001312851.1 NZ_CP093477:c51933-51784 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T238708 NZ_LR890457:777027-777512 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT238708 NZ_CP093477:51977-52038 [Klebsiella pneumoniae]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin