Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4843566..4844212 Replicon chromosome
Accession NZ_CP093465
Organism Klebsiella pneumoniae strain VA681

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag MO412_RS23900 Protein ID WP_002920560.1
Coordinates 4843865..4844212 (-) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag MO412_RS23895 Protein ID WP_002920557.1
Coordinates 4843566..4843865 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO412_RS23875 (MO412_23855) 4839772..4840530 - 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -
MO412_RS23880 (MO412_23860) 4840580..4841410 - 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
MO412_RS23885 (MO412_23865) 4841461..4841790 - 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
MO412_RS23890 (MO412_23870) 4841995..4843503 + 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
MO412_RS23895 (MO412_23875) 4843566..4843865 - 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
MO412_RS23900 (MO412_23880) 4843865..4844212 - 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MO412_RS23905 (MO412_23885) 4844388..4846835 - 2448 WP_002920561.1 glycogen phosphorylase -
MO412_RS23910 (MO412_23890) 4846853..4848286 - 1434 WP_002920564.1 glycogen synthase GlgA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T238669 WP_002920560.1 NZ_CP093465:c4844212-4843865 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T238669 NZ_LR890434:331604-331972 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGTATTACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT238669 WP_002920557.1 NZ_CP093465:c4843865-4843566 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT238669 NZ_LR890434:331386-331607 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCTGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References