Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3742168..3742765 Replicon chromosome
Accession NZ_CP093458
Organism Klebsiella pneumoniae strain VA684

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag MO416_RS18150 Protein ID WP_004142563.1
Coordinates 3742448..3742765 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag MO416_RS18145 Protein ID WP_004142561.1
Coordinates 3742168..3742455 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MO416_RS18115 (MO416_18100) 3738248..3738496 + 249 WP_002893037.1 DUF1158 domain-containing protein -
MO416_RS18120 (MO416_18105) 3738514..3738855 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
MO416_RS18125 (MO416_18110) 3738886..3740001 - 1116 WP_075394661.1 MBL fold metallo-hydrolase -
MO416_RS18130 (MO416_18115) 3740181..3740762 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
MO416_RS18135 (MO416_18120) 3740762..3741130 + 369 WP_002893024.1 MmcQ/YjbR family DNA-binding protein -
MO416_RS18140 (MO416_18125) 3741250..3741903 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
MO416_RS18145 (MO416_18130) 3742168..3742455 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
MO416_RS18150 (MO416_18135) 3742448..3742765 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MO416_RS18155 (MO416_18140) 3742950..3743993 - 1044 WP_075394660.1 DUF2157 domain-containing protein -
MO416_RS18160 (MO416_18145) 3744659..3745525 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
MO416_RS18165 (MO416_18150) 3745634..3747061 + 1428 WP_075394659.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T238645 WP_004142563.1 NZ_CP093458:c3742765-3742448 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T238645 NZ_LR890424:1783952-1784284 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT238645 WP_004142561.1 NZ_CP093458:c3742455-3742168 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT238645 NZ_LR890424:1783694-1783951 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References