Detailed information of TA system TA238632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 15322..15979
• Replicon: plasmid pVA833-165
• Accession: NZ_CP093454
• Organism: Klebsiella pneumoniae strain VA833

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: MO408_RS27580
• Protein ID: WP_000270043.1
• Coordinates: 15629..15979 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MO408_RS27575
• Protein ID: WP_000124640.1
• Coordinates: 15322..15624 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MO408_RS27530 (MO408_27505)	10947..11375	+	429	WP_000591074.1	hypothetical protein	-
MO408_RS27535 (MO408_27510)	11432..11791	+	360	WP_000422768.1	hypothetical protein	-
MO408_RS27540 (MO408_27515)	11791..12237	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
MO408_RS27545 (MO408_27520)	12234..12752	+	519	WP_000210756.1	nitrite reductase	-
MO408_RS27550 (MO408_27525)	12752..12982	+	231	WP_000972663.1	hypothetical protein	-
MO408_RS27555 (MO408_27530)	12969..13826	+	858	WP_001167032.1	hypothetical protein	-
MO408_RS27560 (MO408_27535)	14057..14584	+	528	WP_001236377.1	thermonuclease family protein	-
MO408_RS27565 (MO408_27540)	14642..14914	+	273	WP_001043047.1	HU family DNA-binding protein	-
MO408_RS27570 (MO408_27545)	15002..15295	+	294	WP_001239997.1	chromosome segregation protein ParM	-
MO408_RS27575 (MO408_27550)	15322..15624	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
MO408_RS27580 (MO408_27555)	15629..15979	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MO408_RS27585 (MO408_27560)	16142..16690	+	549	WP_001061574.1	transcriptional regulator	-
MO408_RS27590 (MO408_27565)	17031..17225	+	195	WP_000343597.1	hypothetical protein	-
MO408_RS27595 (MO408_27570)	17236..17607	+	372	WP_000516916.1	hypothetical protein	-
MO408_RS27600 (MO408_27575)	17600..18070	+	471	WP_001281821.1	hypothetical protein	-
MO408_RS27605 (MO408_27580)	18085..18420	-	336	WP_000683476.1	hypothetical protein	-
MO408_RS27610 (MO408_27585)	18517..19005	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
MO408_RS27615 (MO408_27590)	19008..19505	+	498	WP_000062185.1	hypothetical protein	-
MO408_RS27620 (MO408_27595)	19720..20424	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
MO408_RS27625 (MO408_27600)	20489..20668	-	180	Protein_33	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCMY-2 / blaNDM-1	htpB	1..164595	164595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T238632 WP_000270043.1 NZ_CP093454:c15979-15629 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T238632 NZ_LR890417:69614-69901 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT238632 WP_000124640.1 NZ_CP093454:c15624-15322 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT238632 NZ_LR890417:69375-69614 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9